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MB Sample ID: SA100967
Local Sample ID: | WG350_Salt-1 |
Subject ID: | SU001456 |
Subject Type: | Bacteria |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
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Sample Preparation:
Sampleprep ID: | SP001464 |
Sampleprep Summary: | Cell pellets were extracted with 200ul of chilled extraction solvent (80% MetOH in MS grade water containing 1ng/µl PIPES and U13C-U15N-glutamine as internal standard) was added to the pellet, mixed and incubated in ice for 5 minutes for quenching metabolism. This is followed by sonication in water bath for 15 min at 4°C with intermittent vortexing. Metabolites were collected in the supernatant by centrifugation at 14000 rpm for 5 min at 4°C. Previous step was repeated twice by adding 100µl 80% chilled solvent each time to increase metabolite yield and polled extracts were stored in -80°C refrigerator till further analysis. |