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MB Sample ID: SA100975

Local Sample ID:CSH4_Salt-3
Subject ID:SU001456
Subject Type:Bacteria
Subject Species:Escherichia coli
Taxonomy ID:562

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Sample Preparation:

Sampleprep ID:SP001464
Sampleprep Summary:Cell pellets were extracted with 200ul of chilled extraction solvent (80% MetOH in MS grade water containing 1ng/µl PIPES and U13C-U15N-glutamine as internal standard) was added to the pellet, mixed and incubated in ice for 5 minutes for quenching metabolism. This is followed by sonication in water bath for 15 min at 4°C with intermittent vortexing. Metabolites were collected in the supernatant by centrifugation at 14000 rpm for 5 min at 4°C. Previous step was repeated twice by adding 100µl 80% chilled solvent each time to increase metabolite yield and polled extracts were stored in -80°C refrigerator till further analysis.