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MB Sample ID: SA138217

Local Sample ID:Sa_003
Subject ID:SU001711
Subject Type:Plant
Subject Species:Glycine max
Taxonomy ID:3847
Age Or Age Range:6 days

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Sample Preparation:

Sampleprep ID:SP001717
Sampleprep Summary:For metabolomics analysis, frozen hairy roots were ground with liquid nitrogen and extracted in methanol:water (80:20, v/v). The samples were sonicated on ice water bath for 15 min followed by centrifugation at 11,000×g for 10 min at ambient temperature. The supernatant (350 µL) was dried under nitrogen gas. The dried pellet was dissolved in 200 µL of 50% methanol containing 10 µg caffeine as an internal standard and filtered through a 0.45 µm syringe filter.
Sampleprep Protocol ID:jrenaud_SP_Sample_preparation.pdf