Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA202993

Local Sample ID:	Cell-Cis-48h-4
Subject ID:	SU002201
Subject Type:	Cultured cells
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Cell Strain Details:	A2780 ovarian cancer cells

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Analysis
NMR

Sample Preparation:

Sampleprep ID:	SP002207
Sampleprep Summary:	Frozen samples were thawed at room temperature and shaken before use. For cell lysates, 50 μl of 2H2O were added to 450 μl of each lysate sample. In the case of cell culture media, 300 μl of sodium phosphate buffer (70 mM Na2HPO4; 20% v/v 2H2O; 4.6 mM TMSP, pH 7.4) was added to 300 μl of each medium sample. The mixtures were homogenized by vortexing for 30 s and transferred into 5 mm NMR tubes (Bruker BioSpin srl) for analysis.

Sampleprep ID:

SP002207

Sampleprep Summary:

Frozen samples were thawed at room temperature and shaken before use. For cell lysates, 50 μl of 2H2O were added to 450 μl of each lysate sample. In the case of cell culture media, 300 μl of sodium phosphate buffer (70 mM Na2HPO4; 20% v/v 2H2O; 4.6 mM TMSP, pH 7.4) was added to 300 μl of each medium sample. The mixtures were homogenized by vortexing for 30 s and transferred into 5 mm NMR tubes (Bruker BioSpin srl) for analysis.