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MB Sample ID: SA298979
| Local Sample ID: | 29_T1 |
| Subject ID: | SU002893 |
| Subject Type: | Mammal |
| Subject Species: | Sus scrofa |
| Taxonomy ID: | 9823 |
| Age Or Age Range: | ~4 months |
| Weight Or Weight Range: | 35-45 kg |
| Gender: | Male |
| Animal Animal Supplier: | TOPIGS TN70, Tojapigs, the Netherlands |
| Animal Light Cycle: | 12 hours |
| Animal Water: | ad libitum |
| Species Group: | Mammals |
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Sample Preparation:
| Sampleprep ID: | SP002899 |
| Sampleprep Summary: | Samples were extracted in an 80% methanol (80:20 methanol:water) (Methanol ≥99.9%, HiPerSolv CHROMANORM®, ULTRA for LC-MS, suitable for UPLC/UHPLC-MS instruments, VWR, Belgium) extraction buffer containing 1 µM of deuterated D27 myristic acid, 5 µM D12 glucose, 3 µM 13C5-D5-15N Glutamic acid and 3 µM D7-15N4-Arginine as internal standards. 10 µl of sample was added to 990 µl of the extraction buffer and stored overnight at -80 °C. Insolubilities and precipitated proteins were removed by centrifugation at 20.000 g, for 15 min at 4 °C. 200 µL of the supernatant was transferred to an appropriate mass-spectrometry vial. 50 µl of samples was added to 950 µl of the extraction buffer and stored overnight at -80°C. |
| Extract Storage: | -80℃ |
