Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA342880

Local Sample ID:	CNS_RPLC_POS_WT_3
Subject ID:	SU003281
Subject Type:	Insect
Subject Species:	Drosophila melanogaster
Taxonomy ID:	7227
Species Group:	Insects

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP003288
Sampleprep Summary:	Whole larvae and CNS samples were extracted utilizing a tissue homogenizer, employing a solvent mixture of acetonitrile, methanol, and water in a 2:2:1 ratio, using 40 µL per mg of wet weight. The samples were then stored at -20°C overnight. Following centrifugation at 14,000 x g for 10 minutes at 4°C, the supernatant was transferred into an LC/MS vial and subsequently stored at -80°C until LC/MS analysis.

Sampleprep ID:

SP003288

Sampleprep Summary:

Whole larvae and CNS samples were extracted utilizing a tissue homogenizer, employing a solvent mixture of acetonitrile, methanol, and water in a 2:2:1 ratio, using 40 µL per mg of wet weight. The samples were then stored at -20°C overnight. Following centrifugation at 14,000 x g for 10 minutes at 4°C, the supernatant was transferred into an LC/MS vial and subsequently stored at -80°C until LC/MS analysis.