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MB Sample ID: SA378263
| Local Sample ID: | 14 Plasma Fasnall Std 10 ug/ml |
| Subject ID: | SU003563 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | NOD Scid Gamma (NSG) |
| Age Or Age Range: | 3 months |
| Weight Or Weight Range: | 24-33 g |
| Gender: | Female |
| Animal Animal Supplier: | Wistar Animal Facility |
| Animal Housing: | Wistar Animal Facility |
| Animal Feed: | Standard chow diet |
| Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP003570 |
| Sampleprep Summary: | Plasma metabolomics samples were prepared from whole blood mixed with EDTA to 10 mM final concentration. To separate plasma, blood samples were centrifuged at 2,000 g and 4 °C for 10 min. Then, 50 µl of plasma were mixed with 450 µl of 100% methanol to reach 90% final methanol concentration. Tissue metabolite extracts were prepared from ~50 mg snap-frozen tissue samples. Each sample was weighed while frozen. Frozen samples were ground at the temperature of liquid nitrogen by Retch Cryomill. Then, 80% methanol was added to each tube at a ratio of 1 ml per 50 mg of tissue. Regardless of the type of metabolite extracts, all samples were centrifuged at 18,000 g and 4 °C for 20 min. The supernatant was then transferred to new Eppendorf tubes and centrifuged again with the same parameters. After centrifugation, the extracts were transferred to glass LC-MS vials. |
| Sampleprep Protocol Filename: | DM_metabolomics_samples.txt |
| Processing Storage Conditions: | 4℃ |
| Extraction Method: | 80% methanol for organs, 90% for plasma |
| Extract Enrichment: | None |
| Extract Cleanup: | None |
| Extract Storage: | -80℃ |
| Sample Resuspension: | None |
| Sample Derivatization: | None |
| Sample Spiking: | None |
| Subcellular Location: | Intracellular metabolites and medium metabolites |
