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MB Sample ID: SA385794
Local Sample ID: | XiaChaoYi_A6_RP pos |
Subject ID: | SU003633 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | Male |
Species Group: | Mammals |
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Sample Preparation:
Sampleprep ID: | SP003640 |
Sampleprep Summary: | Cells were trypsinized, washed and resuspended in methanol: acetonitrile: ddH2O2 (2:2:1, v/v) after indicated treatment. Then subjected to LC/MS analysis at LipidALL Technologies (Changzhou, China). Briefly, polar metabolites were extracted using 1000 µl of ice-cold methanol: H2O (4:1, v/v), and incubated at 2000 × g for 30 min at 4oC. At the end of the incubation, samples were centrifuged for 10 min at 13,000 × g at 4oC. Clean supernatant was transferred to a new tube. Extracts were dried in a centrifugal concentrator. |
Sampleprep Protocol Filename: | 2024-NC.pdf |