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MB Sample ID: SA100937
Local Sample ID: | NR_PC_NS_OS_604 |
Subject ID: | SU001455 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Treatment:
Treatment ID: | TR001470 |
Treatment Summary: | To investigate the pro-growth changes, we used the a transgenic channelrhodopsin mice (Thy1-ChR2-EYFP mice) in C57BL/6Jas a model of regeneration after optic nerve crush and C57BL/6J mice as control. The Thy1-Chr2-EYFP mouse line, which has the retinal ganglion cell (RGC) expressing channelrhodopsin-2 (Chr2) and enhanced yellow fluorescent protein (EYFP) expression utilizing an internal ribosomal entry site (IRES) within the same promoter, is widely used in optogenetic stimulation studies. The optogenetic stimulation activates Chr2. For the optic nerve crush, a surgical peritomy was made behind and above the eyeball and the eye muscles were gently retracted to expose the optic nerve. Dumont #5 forceps (FST) were used to crush the optic nerve approximately 0.5-1 mm behind the globe without damaging retinal vessels or affecting the blood supply. |