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MB Sample ID: SA186804

Local Sample ID:	8h 3-
Subject ID:	SU002078
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammals

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Treatment:

Treatment ID:	TR002090
Treatment Summary:	LPS-primed murine peritoneal macrophages were fed fluorescently-tagged IgG-opsonized live Jurkat cells for 1h. Unengulfed targets were washed off and macrophages were left to degrade targets for a further 3 or 7 hours. Macrophages were then collected and sorted into non-engulfing or engulfing populations, alongside control unexposed macrophages. Sorted cells were pelleted at 400xg and dry pellets were snap frozen and stored at -80C until metabolite extraction.

Treatment ID:

TR002090

Treatment Summary:

LPS-primed murine peritoneal macrophages were fed fluorescently-tagged IgG-opsonized live Jurkat cells for 1h. Unengulfed targets were washed off and macrophages were left to degrade targets for a further 3 or 7 hours. Macrophages were then collected and sorted into non-engulfing or engulfing populations, alongside control unexposed macrophages. Sorted cells were pelleted at 400xg and dry pellets were snap frozen and stored at -80C until metabolite extraction.