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MB Sample ID: SA245032
Local Sample ID: | 3_9 |
Subject ID: | SU002539 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | Primary microglia isolated from APOE-targeted replacement mice homozygous for human E3 (B6.129P2-Apoe^tm2(APOE*3)Mae N8, Taconic #1548-F) or human E4 (B6.129P2- Apoe^tm3(APOE*4)Mae N8, Taconic #1549-F) alleles |
Age Or Age Range: | P0-P3 |
Gender: | Pooled |
Species Group: | Mammals |
Select appropriate tab below to view additional metadata details:
Treatment:
Treatment ID: | TR002551 |
Treatment Summary: | Cells were not treated, as we were just comparing APOE3/3 vs. APOE4/4 genotypes |
Cell Growth Container: | 6-well plate |
Cell Media: | DMEM/F12 with 10% FBS, 10% LCCM supplement (see full methods in associated publication for preparation details), and 1% Penicillin/Streptomycin |
Cell Envir Cond: | 37C, 5% CO2 |
Cell Harvesting: | Cell scraper |
Cell Pct Confluence: | 100 |
Cell Media Lastchanged: | 2-4 days prior to harvest |