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MB Sample ID: SA378135
| Local Sample ID: | 42 21-1 Fasnall 20 uM Medium 1 |
| Subject ID: | SU003562 |
| Subject Type: | Fish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Gender: | Pooled |
| Species Group: | Fish |
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Treatment:
| Treatment ID: | TR003571 |
| Treatment Summary: | The zebrafish research was approved by the University of Pennsylvania Institutional Animal Care and Use Committee. Husbandry was performed in accordance with institutional animal welfare guidelines. Embryos from wild type, Tübingen zebrafish were collected within 15 minutes of fertilization. Embryos between 2 and 3 crosses were equally pooled and allocated to treatment conditions for all experiments. Embryos were reared at 28 °C in E3 medium (4 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl2, and 0.33 mM MgSO4, no methylene blue) until treatment. Pharmacological agents were diluted from stocks at room temperature in E3 for 15 minutes before treatment. For treatment at 48 h post-fertilization, embryos in their chorions were transferred to clean plates with the inhibitor-supplemented E3 and incubated for 6 hours. |
| Treatment Compound: | Rotenone, Fasnall, TVB-2640, and GSK2194069 |
| Treatment Route: | Drugs were dissolved in the medium |
| Treatment Vehicle: | DMSO |
