Summary of study ST000076

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000073. The data can be accessed directly via it's Project DOI: 10.21228/M8B88S This work is supported by NIH grant, U2C- DK119886.

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Study IDST000076
Study TitleA549 Cell Study
Study Typetracer
Study Summary13C-Glu and 13C/15N-Gln SIRM study of A549 cells
Institute
University of Kentucky
DepartmentSchool of Medicine
Last NameYan
First NameJun
Emailjun.yan@louisville.edu
Submit Date2014-07-02
Num Groups3
Total Subjects6
Raw Data AvailableNo
Analysis Type DetailGC/LC-MS
Release Date2014-11-01
Release Version1
Jun Yan Jun Yan
https://dx.doi.org/10.21228/M8B88S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000073
Project DOI:doi: 10.21228/M8B88S
Project Title:CESB Lung Cancer Project
Project Type:MS/NMR
Project Summary:Influence of Microenvironmental Nutrient Availability on Systems Biochemistry of Lung Cancer Cells and Immuomodulation. This cell-based project aims to gain a systematic understanding of lung cancer metabolism and dysregulations as a function of key microenvironmental factors that govern lung cancer cell development and its ability to evade immune surveillance. The factors to be examined include the availability of key nutrients crucial to cancer proliferation, survival, metastasis, and immunoregulation.
Institute:University of Kentucky
Department:Toxicology
Last Name:Fan
First Name:Teresa
Email:twmfan@gmail.com

Subject:

Subject ID:SU000095
Subject Type:Human cells
Subject Species:Homo sapiens
Taxonomy ID:9606
Genotype Strain:A549
Species Group:Human

Factors:

Subject type: Human cells; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Labeling
SA00396313C/15N-Gln-plate-1-cells13C/15N-Gln
SA00396413C/15N-Gln-plate-2-cells13C/15N-Gln
SA00396113C-Glc-plate-2-cells13C-Glc
SA00396213C-Glc-plate-1-cells13C-Glc
SA003965unlabeled-plate-2-cellsunlabeled
SA003966unlabeled-plate-1-cellsunlabeled
Showing results 1 to 6 of 6

Collection:

Collection ID:CO000078
Collection Summary:cell_quench_collection
Collection Protocol Filename:14Jun17_Fan_Quench_cell_tissue_SOP.pdf
Collection Protocol Comments:Collection and quench of cells in tissue culture
Sample Type:Lung

Treatment:

Treatment ID:TR000096
Treatment Summary:13C-Glc|13C/15N-Gln|unlabeled
Treatment Protocol Filename:14Jun17Fan_Cell_Tracer_Ex_SOP.pdf
Treatment Protocol Comments:Cell culture in 13C-Glc-labeled media | 13C/15N-Gln-labeled media | unlabeled media for 24h

Sample Preparation:

Sampleprep ID:SP000091
Sampleprep Summary:protein_extraction|polar_preparation|polar_extraction
Sampleprep Protocol Filename:14June20Fan_Prot_Quant_SOP.pdf
14Jun30Fan_Prep_Polar_SOP.pdf
14Jun30Fan_Extract_Polar_Lipid_Prot_SOP.pdf
Sampleprep Protocol Comments:Protein extraction and quantification|Preparation of polar samples for GCMS and NMR|Polar extraction from cells.

Combined analysis:

Analysis ID AN000121 AN000122
Analysis type MS MS
Chromatography type GC GC
Chromatography system
Column
MS Type ESI EI
MS instrument type Single quadrupole GC-ITQ
MS instrument name Thermo LTQ-FT Thermo ITQ
Ion Mode NEGATIVE POSITIVE
Units normalized corrected Peak area Peak area

Chromatography:

Chromatography ID:CH000083
Chromatography Type:GC

MS:

MS ID:MS000097
Analysis ID:AN000121
Instrument Name:Thermo LTQ-FT
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:FTMS Analytical Experiment with detection of compounds with unique CHONPS molecular formula
Ion Mode:NEGATIVE
  
MS ID:MS000098
Analysis ID:AN000122
Instrument Name:Thermo ITQ
Instrument Type:GC-ITQ
MS Type:EI
MS Comments:GCMS Analytical Experiment with detection of compounds by comparison to standards. Derivatization is done with MTBSTFA (N-methyl-N-[tert-butyldimethylsilyl]trifluoroacetamide)
Ion Mode:POSITIVE
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