Summary of study ST000107

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000046. The data can be accessed directly via it's Project DOI: 10.21228/M84S36 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000107
Study TitleMetabolomic and lipidomic profiles in response to exogenous insulin and GLP-1 infusions during prolonged fasting
Study TypeTimecourse
Study SummarySeventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes.
Institute
University of California, Davis
DepartmentNutrition
LaboratoryNewman
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2014-07-10
Num Groups4
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s)mzML, .MS, .txt, INI
Uploaded File Size751 M
Analysis Type DetailGC/LC-MS
Release Date2015-02-03
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M84S36
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000046
Project DOI:doi: 10.21228/M84S36
Project Title:Metabolomic & lipidomic profiles in response to exogenous insulin & GLP-1 infusions during prolonged fasting
Project Type:Timecourse
Project Summary:This application requests funding to access state-of-the-art metabolomics and lipidomic platforms at the NIH West Coast Metabolomics Center to analyze plasma samples from recent insulin and glucagon-like peptide-1 (GLP-1) infusion experiments performed in prolong-fasted elephant seals. This suite of studies was designed to better assess the mechanisms contributing to the onset of an insulin resistantlike condition induced by prolonged food deprivation/starvation in mammals. Because elephant seals have evolved robust physiological mechanisms that have allowed them to naturally tolerate such protracted bouts of fasting, they provide an ideal model to address our central hypothesis that increased lipid utilization late in the fast contributes to insulin resistance in elephant seals. Insulin resistance is a common consequence of fasting in mammals and, while the mechanisms by which it manifests are still unclear, a metabolic shift favoring increased mobilization and utilization of lipids during prolonged food deprivation may be a principal causative factor. Insulin resistance has a negative connotation due to its association with obesity and diabetes among humans, but it has been suggested to be an adaptive response to food deprivation.
Institute:University of California, Merced
Department:School of Natural Sciences
Laboratory:Molecular and Cellular Biology, Natural Sciences
Last Name:Ortiz
First Name:Rudy
Address:5200 N. Lake Rd.; Merced, CA 95343
Email:rortiz@ucmerced.edu
Phone:209.228.2964

Subject:

Subject ID:SU000126
Subject Type:Animal
Subject Species:Mirounga angustirostris
Taxonomy ID:9716
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mirounga angustirostris (Factor headings shown in green)

mb_sample_id local_sample_id Treatment Timepoint
SA005921IE05-0Early ITT Infusion Study 0 mins
SA005922IE04-0Early ITT Infusion Study 0 mins
SA005923IE03-0Early ITT Infusion Study 0 mins
SA005924IE02-0Early ITT Infusion Study 0 mins
SA005925IE01-0Early ITT Infusion Study 0 mins
SA005926IE05-10Early ITT Infusion Study 10 mins
SA005927IE04-10Early ITT Infusion Study 10 mins
SA005928IE01-10Early ITT Infusion Study 10 mins
SA005929IE03-10Early ITT Infusion Study 10 mins
SA005930IE02-10Early ITT Infusion Study 10 mins
SA005931IE03-120Early ITT Infusion Study 120 mins
SA005932IE02-120Early ITT Infusion Study 120 mins
SA005933IE04-120Early ITT Infusion Study 120 mins
SA005934IE05-120Early ITT Infusion Study 120 mins
SA005935IE01-120Early ITT Infusion Study 120 mins
SA005936IE01-30Early ITT Infusion Study 30 mins
SA005937IE02-30Early ITT Infusion Study 30 mins
SA005938IE03-30Early ITT Infusion Study 30 mins
SA005939IE04-30Early ITT Infusion Study 30 mins
SA005940IE05-30Early ITT Infusion Study 30 mins
SA005941IE04-60Early ITT Infusion Study 60 mins
SA005942IE01-60Early ITT Infusion Study 60 mins
SA005943IE03-60Early ITT Infusion Study 60 mins
SA005944IE02-60Early ITT Infusion Study 60 mins
SA005948C01-0GLP1/Late GTT 0 mins
SA005949C02-0GLP1/Late GTT 0 mins
SA005950A01-0GLP1/Late GTT 0 mins
SA005951B03-0GLP1/Late GTT 0 mins
SA005952C03-0GLP1/Late GTT 0 mins
SA005953C04-0GLP1/Late GTT 0 mins
SA005954B01-0GLP1/Late GTT 0 mins
SA005955B02-0GLP1/Late GTT 0 mins
SA005956A04-0GLP1/Late GTT 0 mins
SA005957A02-0GLP1/Late GTT 0 mins
SA005958A03-0GLP1/Late GTT 0 mins
SA005959A05-0GLP1/Late GTT 0 mins
SA005960A01-10GLP1/Late GTT 10 mins
SA005961B01-10GLP1/Late GTT 10 mins
SA005962A03-10GLP1/Late GTT 10 mins
SA005963C03-10GLP1/Late GTT 10 mins
SA005964A02-10GLP1/Late GTT 10 mins
SA005965A04-10GLP1/Late GTT 10 mins
SA005966A05-10GLP1/Late GTT 10 mins
SA005967C02-10GLP1/Late GTT 10 mins
SA005968B02-10GLP1/Late GTT 10 mins
SA005969C04-10GLP1/Late GTT 10 mins
SA005970C01-10GLP1/Late GTT 10 mins
SA005971B03-10GLP1/Late GTT 10 mins
SA005972A02-120GLP1/Late GTT 120 mins
SA005973A03-120GLP1/Late GTT 120 mins
SA005974C04-120GLP1/Late GTT 120 mins
SA005975A01-120GLP1/Late GTT 120 mins
SA005976A04-120GLP1/Late GTT 120 mins
SA005977B01-120GLP1/Late GTT 120 mins
SA005978C03-120GLP1/Late GTT 120 mins
SA005979C01-120GLP1/Late GTT 120 mins
SA005980C02-120GLP1/Late GTT 120 mins
SA005981B03-120GLP1/Late GTT 120 mins
SA005982A05-120GLP1/Late GTT 120 mins
SA005983B02-120GLP1/Late GTT 120 mins
SA005984A05-30GLP1/Late GTT 30 mins
SA005985B01-30GLP1/Late GTT 30 mins
SA005986A03-30GLP1/Late GTT 30 mins
SA005987A04-30GLP1/Late GTT 30 mins
SA005988A02-30GLP1/Late GTT 30 mins
SA005989A01-30GLP1/Late GTT 30 mins
SA005990C04-30GLP1/Late GTT 30 mins
SA005991C01-30GLP1/Late GTT 30 mins
SA005992C02-30GLP1/Late GTT 30 mins
SA005993B03-30GLP1/Late GTT 30 mins
SA005994C03-30GLP1/Late GTT 30 mins
SA005995B02-30GLP1/Late GTT 30 mins
SA005996C03-60GLP1/Late GTT 60 mins
SA005997C02-60GLP1/Late GTT 60 mins
SA005998C01-60GLP1/Late GTT 60 mins
SA005999A02-60GLP1/Late GTT 60 mins
SA006000A04-60GLP1/Late GTT 60 mins
SA006001A03-60GLP1/Late GTT 60 mins
SA006002A01-60GLP1/Late GTT 60 mins
SA006003B02-60GLP1/Late GTT 60 mins
SA006004B03-60GLP1/Late GTT 60 mins
SA006005A05-60GLP1/Late GTT 60 mins
SA006006C04-60GLP1/Late GTT 60 mins
SA006007B01-60GLP1/Late GTT 60 mins
SA006008IL05-0Late ITT Infusion Study 0 mins
SA006009IL03-0Late ITT Infusion Study 0 mins
SA006010IL02-0Late ITT Infusion Study 0 mins
SA006011IL01-0Late ITT Infusion Study 0 mins
SA006012IL04-0Late ITT Infusion Study 0 mins
SA006013IL03-10Late ITT Infusion Study 10 mins
SA006014IL04-10Late ITT Infusion Study 10 mins
SA006015IL01-10Late ITT Infusion Study 10 mins
SA006016IL02-10Late ITT Infusion Study 10 mins
SA006017IL05-10Late ITT Infusion Study 10 mins
SA006018IL01-120Late ITT Infusion Study 120 mins
SA006019IL02-120Late ITT Infusion Study 120 mins
SA006020IL05-120Late ITT Infusion Study 120 mins
SA006021IL03-120Late ITT Infusion Study 120 mins
SA006022IL04-120Late ITT Infusion Study 120 mins
SA006023IL05-30Late ITT Infusion Study 30 mins
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Collection:

Collection ID:CO000110
Collection Summary:Following the GLP-1 or insulin infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes.
Collection Protocol Filename:Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf
Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf
Sample Type:Blood
Collection Time:10, 30, 60, 90, and 120 minutes postprandial
Storage Conditions:-80 C
Blood Serum Or Plasma:Plasma

Treatment:

Treatment ID:TR000128
Treatment Summary:Seventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes.
Treatment Protocol Filename:Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf
Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf
Treatment Compound:GLP-1 or Insulin
Treatment Route:Infusion
Treatment Dose:low (10 pmol/kg) or high (100 pmol/kg) dose of GLP-1, insulin (0.065 U/kg)
Animal Fasting:Yes, for insulin study

Sample Preparation:

Sampleprep ID:SP000123
Sampleprep Protocol Filename:Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf
Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf
Processing Storage Conditions:- 20 °C
Extraction Method:SPE
Extract Concentration Dilution:250 µL
Extract Cleanup:SPE
Extract Storage:- 20 °C
Sample Resuspension:100 µL
Sample Spiking:See sample prep protocol file

Combined analysis:

Analysis ID AN000177 AN000178
Analysis type MS MS
Chromatography type GC GC
Chromatography system
Column
MS Type ESI EI
MS instrument type Triple quadrupole Single quadrupole
MS instrument name ABI Sciex API 4000 QTrap Agilent 5973N
Ion Mode POSITIVE POSITIVE
Units nM nM

Chromatography:

Chromatography ID:CH000121
Methods Filename:Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf
Newman_Lab_NEFA_SOPs-Ortiz_WCMC_Pilot.pdf
Column Temperature:60 C
Flow Gradient:See protocol/methods file
Flow Rate:0.25
Internal Standard:See protocol/methods file
Retention Time:See protocol/methods file
Sample Injection:5 L
Solvent A:0.1% acetic acid
Solvent B:90% ACN / 10% IPA
Analytical Time:11 min
Weak Wash Solvent Name:20% methanol, 10% isopropanol
Weak Wash Volume:600 L
Strong Wash Solvent Name:50:50 Acetonitrile:Methanol
Strong Wash Volume:600 L
Sample Loop Size:17 L
Chromatography Type:GC

MS:

MS ID:MS000140
Analysis ID:AN000177
Instrument Name:ABI Sciex API 4000 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:LC/MS
Ion Mode:POSITIVE
Ion Source Temperature:See protocol/methods file
Ion Spray Voltage:See protocol/methods file
  
MS ID:MS000141
Analysis ID:AN000178
Instrument Name:Agilent 5973N
Instrument Type:Single quadrupole
MS Type:EI
MS Comments:LC/MS
Ion Mode:POSITIVE
Ion Source Temperature:See protocol/methods file
Ion Spray Voltage:See protocol/methods file
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