Summary of study ST000240

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000193. The data can be accessed directly via it's Project DOI: 10.21228/M8FK5P This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000240
Study TitleGlobal LC-MS of Ozone Stress in Maize: GLCMS
Study Typeozone treatment
Study SummaryGlobal LC-MS of maize leaf samples collected from plants grown at ambient and elevated ozone concentrations in replicated field experiment
Institute
University of Florida
DepartmentSoutheastern Center for Integrated Metabolomics (SECIM)
Last NameAinsworth
First NameElizabeth
Emailainswort@illinois.edu
Submit Date2015-02-24
Num Groups2
Total Subjects24
Raw Data AvailableYes
Raw Data File Type(s).mzXML
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Elizabeth Ainsworth Elizabeth Ainsworth
https://dx.doi.org/10.21228/M8FK5P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000193
Project DOI:doi: 10.21228/M8FK5P
Project Title:Global LC-MS of Ozone Stress in Maize: GLCMS
Project Summary:Oxidative stress arising from O3 exposure is reducing potential maize yields by up to 10%. Yield losses to O3 are projected to as much as double by mid-century, and there is thought to be little potential for adaptation to rising O3 concentrations ([O3]) through altered crop management practices. Therefore, the only solution to current and future O3-induced yield loss is development of O3 tolerant maize through breeding and/or biotechnology. In this project, we will investigate the metabolite profiles that underpin O3 sensitivity in maize.
Institute:University of Illinois Urbana-Champaign
Department:Plant Biology
Last Name:Ainsworth
First Name:Elizabeth
Address:1201 W. Gregory Dr., Urbana, IL 61801
Email:ainswort@illinois.edu
Phone:(217) 265-9887
Funding Source:Southeastern Center for Integrated Metabolomics (SECIM) pilot and feasibility funding, NIH U24 DK097209

Subject:

Subject ID:SU000260
Subject Type:Plant
Subject Species:Zea mays
Taxonomy ID:4577
Genotype Strain:inbred line B73
Species Group:Plant

Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Ring No Treatment
SA011112R10 B73 4102110 ambient
SA011113R10 B73 4102310 ambient
SA011114R10 B73 4104410 ambient
SA011115R13 B73 4134413 ozone
SA011116R13 B73 4132113 ozone
SA011117R13 B73 4132413 ozone
SA011118R14 B73 4142414 ambient
SA011119R14 B73 4142114 ambient
SA011120R14 B73 4144414 ambient
SA011121R1 B73 401041 ambient
SA011122R1 B73 401431 ambient
SA011123R1 B73 401221 ambient
SA011124R2 B73 402432 ozone
SA011125R2 B73 402222 ozone
SA011126R2 B73 402042 ozone
SA011127R5 B73 405255 ambient
SA011128R5 B73 405425 ambient
SA011129R5 B73 405205 ambient
SA011130R6 B73 406426 ozone
SA011131R6 B73 406256 ozone
SA011132R6 B73 406206 ozone
SA011133R9 B73 409449 ozone
SA011134R9 B73 409219 ozone
SA011135R9 B73 409239 ozone
Showing results 1 to 24 of 24

Collection:

Collection ID:CO000250
Collection Protocol Filename:Collection_summary_ainsworth.txt
Sample Type:Plant

Treatment:

Treatment ID:TR000270
Treatment Summary:Maize (inbred line B73) grown at ambient (~40 ppb) and elevated O3 concentrations ([O3]; 100 ppb).
Treatment Protocol Filename:Treatment_summary_ainsworth.txt

Sample Preparation:

Sampleprep ID:SP000264
Sampleprep Protocol Filename:Appendix_A_-_Internal_Standard_Prep_GLCMS.pdf
Sampleprep Protocol Comments:Appendix A - Internal Standard Prep GLCMS.pdf

Combined analysis:

Analysis ID AN000371 AN000372
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 Thermo Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE
Units Peak height Peak height

Chromatography:

Chromatography ID:CH000269
Methods Filename:Metabolomics_LCMSProtocol.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase

MS:

MS ID:MS000317
Analysis ID:AN000371
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:POSITIVE
  
MS ID:MS000318
Analysis ID:AN000372
Instrument Name:Thermo Q Exactive Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
Ion Mode:NEGATIVE
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