Summary of Study ST001206

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000811. The data can be accessed directly via it's Project DOI: 10.21228/M8VD62 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001206
Study Title	Effects of cold exposure on serum lipidomic in mice
Study Summary	We aimed to identify lipids released during cold exposure from adipose tissue, with a role in adaptive thermogenesis.
Institute	Joslin Diabetes Center
Department	Integrative Physiology and Metabolism
Laboratory	Yu-Hua Tseng lab
Last Name	Leiria
First Name	Luiz
Address	One Joslin Place, Boston-MA, USA, 02215
Email	luiz.leiria@joslin.harvard.edu
Phone	1 6173091967
Submit Date	2019-06-26
Study Comments	Joslin Diabetes Center affiliate of Harvard Medical School
Raw Data Available	Yes
Raw Data File Type(s)	wiff
Analysis Type Detail	LC-MS
Release Date	2019-07-17
Release Version	1

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Project:

Project ID:	PR000811
Project DOI:	doi: 10.21228/M8VD62
Project Title:	12-LOX metabolites in cold adaptation
Project Summary:	The goal of this project is to understand the role of the enzyme 12-lipoxygenase in the adaptive thermogenesis. We found this enzyme is activated by cold stimulation, then producing lipid metabolites in adipose tissue and releasing them into the circulation to regulate fuel utilisation and thermogenic pathways required for the cold adaptation.
Institute:	Joslin Diabetes Center
Department:	Integrative Physiology and Metabolism
Laboratory:	Yu-Hua Tseng lab
Last Name:	Leiria
First Name:	Luiz
Address:	One Joslin Place, Boston, MA-USA, 02215
Email:	luiz.leiri@joslin.harvard.edu
Phone:	1 6173091967

Subject:

Subject ID:	SU001273
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id	local_sample_id	Temperature	Time of exposure
SA084770	Control 1	22C	1 hour
SA084771	Control 2	22C	1 hour
SA084772	Control 5	22C	1 hour
SA084773	Control 6	22C	1 hour
SA084774	Control 4	22C	1 hour
SA084775	Control 3	22C	1 hour
SA084776	Thermoneutral 2 female	30C	7 days
SA084777	Thermoneutral 1 female	30C	7 days
SA084778	Thermoneutral 3 female	30C	7 days
SA084779	Thermoneutral 6 female	30C	7 days
SA084780	Thermoneutral 5 male	30C	7 days
SA084781	Thermoneutral 5 female	30C	7 days
SA084782	Thermoneutral 4 female	30C	7 days
SA084783	Thermoneutral 6 male	30C	7 days
SA084784	Thermoneutral 4 male	30C	7 days
SA084785	Thermoneutral 1 male	30C	7 days
SA084786	Thermoneutral 2 male	30C	7 days
SA084787	Thermoneutral 3 male	30C	7 days
SA084788	1-hr cold 1	5C	1 hour
SA084789	1-hr cold 2	5C	1 hour
SA084790	1-hr cold 3	5C	1 hour
SA084791	1-hr cold 4	5C	1 hour
SA084792	1-hr cold 5	5C	1 hour
SA084793	Cold 3 female	5C	7 days
SA084794	Cold 4 female	5C	7 days
SA084795	Cold 5 female	5C	7 days
SA084796	Cold 2 female	5C	7 days
SA084797	Cold 2 male	5C	7 days
SA084798	Cold 1 male	5C	7 days
SA084799	Cold 3 male	5C	7 days
SA084800	Cold 4 male	5C	7 days
SA084801	Cold 5 male	5C	7 days
SA084802	Cold 1 female	5C	7 days

Showing results 1 to 33 of 33

Showing results 1 to 33 of 33

Collection:

Collection ID:	CO001267
Collection Summary:	Mice were anaesthethized with isoflurane, the blood collected through cardiac puncture, and left under room temperature for 30 minutes. After that, the blood was centrifuged for 3 minutes at 14,000 rpm. Then, serum was transferred for a clean 1.5ml microbe and frost.
Sample Type:	Blood (serum)
Storage Conditions:	-20℃

Treatment:

Treatment ID:	TR001288
Treatment Summary:	We divided the C57BL6/J mice in 6 groups: females housed in 30C degree for 1 week and females housed in 5C degree for 1 week; Males housed in 30C degree for 1 week and females housed in 5C degree for 1 week; males housed in 22C for 1 hour and males housed in 5C for 1 hour.

Sample Preparation:

Sampleprep ID:	SP001281
Sampleprep Summary:	Aliquots of 100 µL serum or were taken. A mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform.

Sampleprep ID:

SP001281

Sampleprep Summary:

Aliquots of 100 µL serum or were taken. A mixture of deuterium-labeled internal standards was added to each aliquot, followed by 3x volume of sample of cold methanol (MeOH). Samples were vortexed for 5 minutes and stored at −20 °C overnight. Cold samples were centrifuged at 14,000g for 10 minutes, and the supernatant was then transferred to a new tube and 3 mL of acidified H2O (pH 3.5) was added to each sample prior to C18 SPE. The methyl formate fractions were collected, dried under nitrogen, and reconstituted in 50 µL MeOH:H2O (1:1, by vol). Samples were transferred to 0.5 mL tubes and centrifuged at 20,000g at 4 °C for 10 minutes. (35ul) of supernatant was transferred to LC–MS/MS vials for analysis using the BERG LC–MS/MS mediator lipidomics platform.

Combined analysis:

Analysis ID	AN002008
Analysis type	MS
Chromatography type	Reversed phase
Chromatography system	Ekspert MicroLC 200 system
Column	Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um)
MS Type	ESI
MS instrument type	QTOF
MS instrument name	ABI Sciex 5600+ TripleTOF
Ion Mode	NEGATIVE
Units	Peak Area

Chromatography:

Chromatography ID:	CH001452
Instrument Name:	Ekspert MicroLC 200 system
Column Name:	Phenomenex Synergi Fusion-RP capillary C18 (150 × 0.5 mm,4um)
Chromatography Type:	Reversed phase

MS:

MS ID:	MS001861
Analysis ID:	AN002008
Instrument Name:	ABI Sciex 5600+ TripleTOF
Instrument Type:	QTOF
MS Type:	ESI
MS Comments:	MS spectra were acquired in high-resolution mode (>30,000) using a 100-ms accumulation time per spectrum. Full-scan MS/MS was acquired in high sensitivity mode, with an accumulation time optimized per cycle. Collision energy was set using rolling collision energy with a spread of 15V. The identity of a component was confirmed using PeakView® software (SCIEX), and quantification was performed using MultiQuant™ software (SCIEX).
Ion Mode:	NEGATIVE