Summary of Study ST001292

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000874. The data can be accessed directly via it's Project DOI: 10.21228/M8Q975 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001292
Study TitleMetabolomics of blood plasma and kidney tissue from control (db/m) and diabetic (db/db) mice.
Study TypeMS/MSMS analysis
Study SummaryPlasma and kidney from control (db/m) and diabetic (db/db) mice on regular or eNOS -/- background. Some mice received treatment (20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age). Samples paired (plasma/tissue from individual mice). Samples snap frozen and stored at -80 until use.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address1000 wall St., Ann Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-12-16
Study CommentsThe wrong mouse number was recorded for P_29 and P_59 so they were excluded from analysis.
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2019-12-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8Q975
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000874
Project DOI:doi: 10.21228/M8Q975
Project Title:Metabolomics of blood plasma and kidney tissue from control (db/m) and diabetic (db/db) mice.
Project Type:MS/MSMS analysis
Project Summary:Determine lipids in plasma and microvascular complication prone tissues in diabetic mouse models
Institute:Michigan Center for Translational Pathology
Department:UMich Internal Medicine
Laboratory:Pennathur Lab
Last Name:Pennathur
First Name:Subramaniam
Address:University Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105
Email:spennath@umich.edu
Phone:734 615 5757

Subject:

Subject ID:SU001366
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammals

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id ACE/ARB Inhibitor control/diabetic eNOS background
SA093900S00030150No Treat db/db diabetic eNOS +/+
SA093901S00030144No Treat db/db diabetic eNOS +/+
SA093902S00030128No Treat db/db diabetic eNOS +/+
SA093903S00030157No Treat db/db diabetic eNOS +/+
SA093904S00030166No Treat db/db diabetic eNOS +/+
SA093905S00030173No Treat db/db diabetic eNOS +/+
SA093906S00030171No Treat db/db diabetic eNOS +/+
SA093907S00030121No Treat db/db diabetic eNOS +/+
SA093908S00030164No Treat db/db diabetic eNOS +/+
SA093909S00030136No Treat db/db diabetic eNOS +/+
SA093910S00030167No Treat db/db diabetic eNOS -/-
SA093911S00030117No Treat db/db diabetic eNOS -/-
SA093912S00030152No Treat db/db diabetic eNOS -/-
SA093913S00030131No Treat db/db diabetic eNOS -/-
SA093914S00030159No Treat db/db diabetic eNOS -/-
SA093915S00030098No Treat db/db diabetic eNOS -/-
SA093916S00030146No Treat db/db diabetic eNOS -/-
SA093917S00030139No Treat db/db diabetic eNOS -/-
SA093918S00030110No Treat db/db diabetic eNOS -/-
SA093919S00030170No Treat db/db diabetic eNOS -/-
SA093920S00030129No Treat db/m control eNOS +/+
SA093921S00030158No Treat db/m control eNOS +/+
SA093922S00030151No Treat db/m control eNOS +/+
SA093923S00030137No Treat db/m control eNOS +/+
SA093924S00030145No Treat db/m control eNOS +/+
SA093925S00030103No Treat db/m control eNOS +/+
SA093926S00030108No Treat db/m control eNOS +/+
SA093927S00030115No Treat db/m control eNOS +/+
SA093928S00030165No Treat db/m control eNOS +/+
SA093929S00030122No Treat db/m control eNOS +/+
SA093930S00030119No Treat db/m control eNOS -/-
SA093931S00030099No Treat db/m control eNOS -/-
SA093932S00030172No Treat db/m control eNOS -/-
SA093933S00030112No Treat db/m control eNOS -/-
SA093934S00030154No Treat db/m control eNOS -/-
SA093935S00030141No Treat db/m control eNOS -/-
SA093936S00030161No Treat db/m control eNOS -/-
SA093937S00030133No Treat db/m control eNOS -/-
SA093938S00030125No Treat db/m control eNOS -/-
SA093939S00030105No Treat db/m control eNOS -/-
SA093940S00030148Treat db/db diabetic eNOS +/+
SA093941S00030162Treat db/db diabetic eNOS +/+
SA093942S00030168Treat db/db diabetic eNOS +/+
SA093943S00030102Treat db/db diabetic eNOS +/+
SA093944S00030155Treat db/db diabetic eNOS +/+
SA093945S00030142Treat db/db diabetic eNOS +/+
SA093946S00030126Treat db/db diabetic eNOS +/+
SA093947S00030134Treat db/db diabetic eNOS +/+
SA093948S00030113Treat db/db diabetic eNOS +/+
SA093949S00030106Treat db/db diabetic eNOS +/+
SA093950S00030116Treat db/db diabetic eNOS -/-
SA093951S00030130Treat db/db diabetic eNOS -/-
SA093952S00030174Treat db/db diabetic eNOS -/-
SA093953S00030169Treat db/db diabetic eNOS -/-
SA093954S00030138Treat db/db diabetic eNOS -/-
SA093955S00030109Treat db/db diabetic eNOS -/-
SA093956S00030123Treat db/db diabetic eNOS -/-
SA093957S00030127Treat db/m control eNOS +/+
SA093958S00030114Treat db/m control eNOS +/+
SA093959S00030120Treat db/m control eNOS +/+
SA093960S00030163Treat db/m control eNOS +/+
SA093961S00030156Treat db/m control eNOS +/+
SA093962S00030149Treat db/m control eNOS +/+
SA093963S00030143Treat db/m control eNOS +/+
SA093964S00030107Treat db/m control eNOS +/+
SA093965S00030135Treat db/m control eNOS +/+
SA093966S00030101Treat db/m control eNOS +/+
SA093967S00030111Treat db/m control eNOS -/-
SA093968S00030100Treat db/m control eNOS -/-
SA093969S00030160Treat db/m control eNOS -/-
SA093970S00030140Treat db/m control eNOS -/-
SA093971S00030147Treat db/m control eNOS -/-
SA093972S00030153Treat db/m control eNOS -/-
SA093973S00030132Treat db/m control eNOS -/-
SA093974S00030124Treat db/m control eNOS -/-
SA093975S00030104Treat db/m control eNOS -/-
SA093976S00030118Treat db/m control eNOS -/-
Showing results 1 to 77 of 77

Collection:

Collection ID:CO001361
Collection Summary:Kidney samples snap-frozen and stored at -80 until use.
Sample Type:Kidney
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001381
Treatment Summary:Treatment group mice received 20 mg/kg/d lisinopril + 30 mg/kg/d losartan) for 12 wk (starting at 12 wk age), controls didn't receive any drugs.

Sample Preparation:

Sampleprep ID:SP001374
Sampleprep Summary:Standard tissue lipidomics protocol
Sampleprep Protocol Filename:A004_-_Shotgun_Lipidomics.pdf

Combined analysis:

Analysis ID AN002149 AN002150
Chromatography ID CH001572 CH001573
MS ID MS002000 MS002001
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Shimadzu Nexera X2 Shimadzu Nexera X2
Column Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um) Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
MS Type ESI ESI
MS instrument type Triple TOF Triple TOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH001572
Instrument Name:Shimadzu Nexera X2
Column Name:Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH001573
Instrument Name:Shimadzu Nexera X2
Column Name:Waters Acquity BEH HSS T3 (50 x 2.1mm,1.8um)
Chromatography Type:Reversed phase

MS:

MS ID:MS002000
Analysis ID:AN002149
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids
Ion Mode:POSITIVE
  
MS ID:MS002001
Analysis ID:AN002150
Instrument Name:ABI Sciex 5600+ TripleTOF
Instrument Type:Triple TOF
MS Type:ESI
MS Comments:MSMS acquisition followed by LipidBLAST identification and targeted data extraction from MS1 trace for identified lipids
Ion Mode:NEGATIVE
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