Summary of study ST001301

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000882. The data can be accessed directly via it's Project DOI: 10.21228/M8PD88 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001301
Study TitleLuminal metabolome profiles of human microbiota-associated (HMA) mice treated with anti-IL-22 antibody or control antibody
Study SummaryLuminal (fecal) metabolome of anti-IL-22 antibody-treated HMA mice and control antibody-treated HMA mice were compared.
Institute
University of Michigan
DepartmentInternal Medicine
Last NameKamada
First NameNobuhiko
Address1150 W Medical center Dr, Ann Arbor, MI 48109, USA
Emailnkamada@umich.edu
Phone+1-734-763-2142
Submit Date2019-12-22
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2020-01-08
Release Version1
Nobuhiko Kamada Nobuhiko Kamada
https://dx.doi.org/10.21228/M8PD88
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000882
Project DOI:doi: 10.21228/M8PD88
Project Title:Luminal metabolome profiles of human microbiota-associated (HMA) mice treated with anti-IL-22 antibody or control antibody
Project Summary:We found that healthy (HC)-HMA mice, GF mice were colonized with gut microbiota derived from human HC, were protected from Clostridioides difficile (C. difficile) infection. However, the colonization resistance of HC-HMA mice was abolished by treatment of anti-IL-22 antibody, unlike control antibody-treated HC-HMA mice. Additionally, the gut microbial composition of anti-IL22 antibody-treated HC-HMA mice, C. difficile-susceptible mice, were differ from that of control antibody, thereby it appears that luminal metabolome is changed between 2 groups. To examine the change in the luminal metabolites, fecal samples were collected from anti-IL-22 /control -antibody treated HC-HMA mice, and metabolite profiles of them were analyzed.
Institute:University of Michigan
Department:Internal Medicine
Last Name:Kamada
First Name:Nobuhiko
Address:1150 W Medical center Dr, Ann Arbor, MI 48109, USA
Email:nkamada@umich.edu
Phone:+1-734-763-2142

Subject:

Subject ID:SU001375
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Factor
SA094259HC-HMA-Rag1KO+aIL-22 Ab #13Anti-IL-22 antibody
SA094260HC-HMA-Rag1KO+aIL-22 Ab #14Anti-IL-22 antibody
SA094261HC-HMA-Rag1KO+aIL-22 Ab #12Anti-IL-22 antibody
SA094262HC-HMA-Rag1KO+aIL-22 Ab #11Anti-IL-22 antibody
SA094263Ab6Anti-IL-22 antibody
SA094264HC-HMA-Rag1KO+aIL-22 Ab #15Anti-IL-22 antibody
SA094265HC-Rag1KO+aIL-22l Ab #11Anti-IL-22 antibody
SA094266HC-Rag1KO+aIL-22l Ab #15Anti-IL-22 antibody
SA094267HC-Rag1KO+aIL-22l Ab #14Anti-IL-22 antibody
SA094268HC-Rag1KO+aIL-22l Ab #13Anti-IL-22 antibody
SA094269HC-Rag1KO+aIL-22l Ab #12Anti-IL-22 antibody
SA094270Ab5Anti-IL-22 antibody
SA094271Ab4Anti-IL-22 antibody
SA094272HC-HMA-Rag1KO+Ctrl Ab #7Control antibody
SA094273HC-HMA-Rag1KO+Ctrl Ab #8Control antibody
SA094274HC-HMA-Rag1KO+Ctrl Ab #6Control antibody
SA094275Ab3Control antibody
SA094276Ab2Control antibody
SA094277HC-HMA-Rag1KO+Ctrl Ab #9Control antibody
SA094278HC-HMA-Rag1KO+Ctrl Ab #10Control antibody
SA094279HC-Rag1KO+Ctrl Ab #9Control antibody
SA094280HC-Rag1KO+Ctrl Ab #10Control antibody
SA094281HC-Rag1KO+Ctrl Ab #8Control antibody
SA094282HC-Rag1KO+Ctrl Ab #7Control antibody
SA094283HC-Rag1KO+Ctrl Ab #6Control antibody
SA094284Ab1Control antibody
Showing results 1 to 26 of 26

Collection:

Collection ID:CO001370
Collection Summary:Feces of anti-IL-22 antibody/control antibody-treated HC-HMA mice were harvested.
Sample Type:Feces

Treatment:

Treatment ID:TR001390
Treatment Summary:GF C57BL/6 mice were colonized with HC microbiotas for 2 weeks. After colonization of HC microbiota, the mice were treated with control or anti-IL-22 antibody twice (day -5 and day -3) before collecting fecal samples.

Sample Preparation:

Sampleprep ID:SP001383
Sampleprep Summary:Fecal samples were lyophilized using a VD-800R lyophilizer (TAITEC) for 24 hours. Freeze-dried feces were disrupted with 3.0-mm Zirconia Beads (Biomedical Science) by vigorous shaking (1,500 rpm for 10 min) using Shake Master (Biomedical Science). Fecal metabolites were extracted using the methanol:chloroform:water extraction protocol.

Combined analysis:

Analysis ID AN002166 AN002167
Analysis type MS MS
Chromatography type Other Other
Chromatography system Agilent 7100 CE Agilent 7100 CE
Column Fused-silica, i.d. 50 µm(cation) COSMO(+), i.d.50 μm(anion
MS Type ESI ESI
MS instrument type Other Other
MS instrument name Agilent G3250AA LC/MSD TOF system Agilent G3250AA LC/MSD TOF system
Ion Mode POSITIVE NEGATIVE
Units nmol/g nmol/g

Chromatography:

Chromatography ID:CH001584
Instrument Name:Agilent 7100 CE
Column Name:Fused-silica, i.d. 50 µm(cation)
Internal Standard:20 μM of methionine sulfone
Chromatography Type:Other
  
Chromatography ID:CH001585
Instrument Name:Agilent 7100 CE
Column Name:COSMO(+), i.d.50 μm(anion
Internal Standard:20 μM of D-camphor-10-sulfonic acid (CSA)
Chromatography Type:Other

MS:

MS ID:MS002015
Analysis ID:AN002166
Instrument Name:Agilent G3250AA LC/MSD TOF system
Instrument Type:Other
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:POSITIVE
  
MS ID:MS002016
Analysis ID:AN002167
Instrument Name:Agilent G3250AA LC/MSD TOF system
Instrument Type:Other
MS Type:ESI
MS Comments:In-house software (MasterHands) was used for peak area integration and annotation.
Ion Mode:NEGATIVE
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