Summary of Study ST001525

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001026. The data can be accessed directly via it's Project DOI: 10.21228/M82T3X This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001525
Study TitlePerfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) alter the blood lipidome and the hepatic proteome in a murine model of diet-induced obesity
Study SummaryPerfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) alter the blood lipidome and the hepatic proteome in a murine model of diet-induced obesity
Institute
University of Rhode Island;University of Georgia
DepartmentPharmaceutical and Biomedical Sciences
LaboratoryCummings/Slitt
Last NameIngram
First NameLishann
Address250 West Green Street Athens, GA 30605
Emailingram@carnegiescience.edu
Phone706-542-3792
Submit Date2020-07-30
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2020-12-01
Release Version1
Lishann Ingram Lishann Ingram
https://dx.doi.org/10.21228/M82T3X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001026
Project DOI:doi: 10.21228/M82T3X
Project Title:Perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) alter the blood lipidome and the hepatic proteome in a murine model of diet-induced obesity
Project Type:Lipidomics
Project Summary:Perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic acid (PFHxS) alter the blood lipidome and the hepatic proteome in a murine model of diet-induced obesity
Institute:University of Rhode Island;University of Georgia
Department:Pharmaceutical and Biomedical Sciences
Laboratory:Cummings/Slitt
Last Name:Ingram;Cummings
First Name:Lishann;Brian
Address:250 West Green Street
Email:ingram@carnegiescience.edu;briansc@uga.edu
Phone:706-542-3792
Funding Source:NIEHS;DOD

Subject:

Subject ID:SU001599
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6
Age Or Age Range:8 weeks
Gender:Male and female
Animal Animal Supplier:Jackson Labs (Bar Harbor, ME USA)

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Diet Treatment
SA128454S22HFD none
SA128455S21HFD none
SA128456S24HFD none
SA128457S20HFD none
SA128458S23HFD none
SA128459S19HFD none
SA128442S32HFD PFHxS
SA128443S31HFD PFHxS
SA128444S33HFD PFHxS
SA128445S34HFD PFHxS
SA128446S35HFD PFHxS
SA128447S36HFD PFHxS
SA128448S26HFD PFOS
SA128449S25HFD PFOS
SA128450S27HFD PFOS
SA128451S30HFD PFOS
SA128452S28HFD PFOS
SA128453S29HFD PFOS
SA128472S3LFD none
SA128473S2LFD none
SA128474S4LFD none
SA128475S6LFD none
SA128476S1LFD none
SA128477S5LFD none
SA128460S14LFD PFHxS
SA128461S13LFD PFHxS
SA128462S16LFD PFHxS
SA128463S15LFD PFHxS
SA128464S17LFD PFHxS
SA128465S18LFD PFHxS
SA128466S8LFD PFOS
SA128467S7LFD PFOS
SA128468S9LFD PFOS
SA128469S11LFD PFOS
SA128470S10LFD PFOS
SA128471S12LFD PFOS
Showing results 1 to 36 of 36

Collection:

Collection ID:CO001594
Collection Summary:The study investigated diet-PFAS interactions and the impact of perfluorooctanesulfonic acid (PFOS) and perfluorohexanesulfonic (PFHxS) on the hepatic proteome and blood lipidomic profiles. The results supported the hypothesis that PFOS and PFHxS increase the risk of metabolic and inflammatory disease induced by diet.
Sample Type:Blood (whole)
Storage Conditions:-80℃

Treatment:

Treatment ID:TR001614
Treatment Summary:The mice were fed either a 10.5% kcal, low fat diet (LFD) (D12328, Research Diets, New Brunswick), or a 58% kcal, high fat diet (HFD) (D12331, Research Diets, New Brunswick). The mice were assigned to either diet alone, as controls, or to diet containing 0.0003% PFOS or 0.0003% PFHxS. The resulting treatment groups were as follows: low fat diet (LFD), high fat high carbohydrate diet (HFHC), LFD + PFOS (LPFNA), HFHC + PFOS (HPFOS), LFD + PFHxS (LPFHxS), and HFHC + PFHxS (HPFHxS) at n = 6 per treatment group.

Sample Preparation:

Sampleprep ID:SP001607
Sampleprep Summary:Blood lipids were isolated for lipidomic analysis according to the Bligh and Dyer method (Bligh and Dyer 1959). The lipidomics was performed at the University of Georgia (Athens, GA). Briefly, blood samples designated for lipidomics were suspended in 1.25 ml of methanol and 1.25 ml of chloroform. Tubes were vortexed for 30 s, allowed to sit for 10 min on ice, centrifuged (300 x g; 5 min), and the bottom chloroform layer was transferred to a new test tube. The extraction steps were repeated three times and the chloroform layer combined. A commercial mix of SPLASH Lipidomix internal standards (Avanti Polar Lipids, Inc.) were spiked into each sample. SPLASH Lipidomix Mass Spec standards includes all major lipid classes at ratios similar to that found in human plasma. The collected chloroform layers were dried under nitrogen, reconstituted with 50 µl of methanol: chloroform (3:1 v/v), and stored at 80ºC until analysis. Lipid content was quantified by determining the level of inorganic phosphorus using the Bartlett Assay (Bartlett 1959).

Combined analysis:

Analysis ID AN002546
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo-Fisher LTQ Orbitrap Elite
Column Bruker Micron Magic nanoC18 (130mm X 100um,5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Ion Mode POSITIVE
Units Normalized Peak Height

Chromatography:

Chromatography ID:CH001864
Chromatography Comments:nanoC18 column (length, 130 mm; i.d., 100 μm; particle size, 5 μm; pore size, 150 Å; max flow rate, 500 nL/min; packing material, Bruker Micron Magic 18)
Instrument Name:Thermo-Fisher LTQ Orbitrap Elite
Column Name:Bruker Micron Magic nanoC18 (130mm X 100um,5um)
Flow Rate:450-500 nL/min
Injection Temperature:7 °C
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Time Program:60 mins
Target Sample Temperature:7 °C
Chromatography Type:Reversed phase

MS:

MS ID:MS002364
Analysis ID:AN002546
Instrument Name:Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap
Instrument Type:Orbitrap
MS Type:ESI
MS Comments:Lipid structures were identified based on the retention time and subsequent MS/MS spectra. Essentially, we determined structural information through LC-MS/MS and normalization of available lipid standards using LipidMatch. First, lipidomics data processed lipid features using MZmine as described in (Koelmel et al. 2017). Features observed in the blanks were removed using the blank feature filtration method (Patterson et al. 2017). The blank feature filtration method compared to various other filtering methods has been shown to increase the removal of true negatives while decreasing the removal of true positives (Patterson et al. 2017). The resulting MZmine features were annotated using LipidMatch (Koelmel et al. 2017). These annotations are putative, as annotations are based on in-silico MS/MS spectral libraries without matching internal standards for validation and without confirmation using orthogonal approaches (Sumner et al. 2007). The lipid match program then provided a single point calibration using exogenous lipid internal calibrant that best represents the lipid feature (based on lipid class, adduct and retention time). An R script was applied that combined multiple lipid features (adducts) into one feature 4 representing a unique lipid molecule. All open source lipidomics tools are published and available at http://secim.ufl.edu/ secim-tools/.
Ion Mode:POSITIVE
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