Summary of Study ST001947

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001234. The data can be accessed directly via it's Project DOI: 10.21228/M86H5T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001947
Study TitleMetabolite profiling of shoots of juvenile maize plants
Study TypeMetabolic diversity analysis
Study SummaryProfiles of primary metabolites in the shoots of juvenile maize inbred lines in the Goodman association panel were analyzed by GC-TOFMS to identify genetic components associated with metabolic control and plant performance. The samples also include those from landrace lines and maize wild relatives.
Institute
University of Nebraska-Lincoln
DepartmentBiochemistry
LaboratoryObata
Last NameObata
First NameToshihiro
Address1901 Vine Street, Lincoln, NE 68588
Emailtobata2@unl.edu
Phone(402)472-3762
Submit Date2021-10-13
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailGC-MS
Release Date2022-04-13
Release Version1
Toshihiro Obata Toshihiro Obata
https://dx.doi.org/10.21228/M86H5T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001234
Project DOI:doi: 10.21228/M86H5T
Project Title:Metabolic diversity of the maize inbred lines
Project Summary:Profiles of primary metabolites in the shoots of juvenile maize inbred lines in the Goodman association panel were analyzed by GC-TOFMS to identify genetic components associated with metabolic control and plant performance. The samples also include those from landrace lines and maize wild relatives.
Institute:University of Nebraska-Lincoln
Department:Biochemistry
Laboratory:Obata
Last Name:Obata
First Name:Toshihiro
Address:1901 Vine Street, Lincoln, Nebraska, 68588, USA
Email:tobata2@unl.edu
Phone:(402)472-3762
Funding Source:NSF 1557417
Contributors:Toshihiro Obata, Jinliang Yang

Subject:

Subject ID:SU002025
Subject Type:Plant
Subject Species:Zea mays
Taxonomy ID:4577
Age Or Age Range:17 day after germination

Factors:

Subject type: Plant; Subject species: Zea mays (Factor headings shown in green)

mb_sample_id local_sample_id Pedigree
SA183531MGH64Ames 10248
SA183532MGH8Ames 12729
SA183533MGH20Ames 12731
SA183534MGH45Ames 12734
SA183535MGH76Ames 12735
SA183536MGH118Ames 19000
SA183537MGH228Ames 19016
SA183538MGH213Ames 19284
SA183539MGH243Ames 19288
SA183540MGH56Ames 19293
SA183541MGH236Ames 19308
SA183542MGH16Ames 19309
SA183543MGH158Ames 19313
SA183544MGH98Ames 19314
SA183545MGH60Ames 19316
SA183546MGH138Ames 19319
SA183547MGH196Ames 19323
SA183548MGH27Ames 19326
SA183549MGH97Ames 19328
SA183550MGH182Ames 19330
SA183551MGH161Ames 20140
SA183552MGH178Ames 20190
SA183553MGH85Ames 21793
SA183554MGH13Ames 21796
SA183555MGH237Ames 21797
SA183556MGH256Ames 21798
SA183557MGH94Ames 21799
SA183558MGH28Ames 21800
SA183559MGH121Ames 21801
SA183560MGH107Ames 21802
SA183561MGH176Ames 21803
SA183562MGH246Ames 21808
SA183563MGH81Ames 21809
SA183564MGH230Ames 21810
SA183565MGH150Ames 21814
SA183566MGH69Ames 21826
SA183567MGH111Ames 21828
SA183568MGH280Ames 21829
SA183569MGH54Ames 21830
SA183570MGH261Ames 21851
SA183571MGH136Ames 21852
SA183572MGH113Ames 21856
SA183573MGH181Ames 21860
SA183574MGH101Ames 22440
SA183575MGH172Ames 22443
SA183576MGH75Ames 22748
SA183577MGH9Ames 22754
SA183578MGH266Ames 22763
SA183579MGH152Ames 23405
SA183580MGH159Ames 23450
SA183581MGH190Ames 23503
SA183582MGH65Ames 24610
SA183583MGH14Ames 24734
SA183584MGH252Ames 24752
SA183585MGH194Ames 24989
SA183586MGH272Ames 26116
SA183587MGH91Ames 26604
SA183588MGH221Ames 26771
SA183589MGH72Ames 26808
SA183590MGH83Ames 27064
SA183591MGH222Ames 27065
SA183592MGH26Ames 27066
SA183593MGH122Ames 27067
SA183594MGH233Ames 27068
SA183595MGH219Ames 27069
SA183596MGH187Ames 27070
SA183597MGH130Ames 27071
SA183598MGH164Ames 27072
SA183599MGH151Ames 27074
SA183600MGH183Ames 27078
SA183601MGH59Ames 27079
SA183602MGH126Ames 27080
SA183603MGH55Ames 27081
SA183604MGH186Ames 27082
SA183605MGH7Ames 27085
SA183606MGH47Ames 27086
SA183607MGH184Ames 27087
SA183608MGH58Ames 27088
SA183609MGH10Ames 27089
SA183610MGH248Ames 27090
SA183611MGH239Ames 27091
SA183612MGH25Ames 27094
SA183613MGH5Ames 27095
SA183614MGH110Ames 27097
SA183615MGH265Ames 27100
SA183616MGH255Ames 27102
SA183617MGH278Ames 27104
SA183618MGH153Ames 27105
SA183619MGH99Ames 27106
SA183620MGH227Ames 27107
SA183621MGH38Ames 27108
SA183622MGH66Ames 27110
SA183623MGH244Ames 27111
SA183624MGH119Ames 27112
SA183625MGH79Ames 27114
SA183626MGH250Ames 27116
SA183627MGH105Ames 27118
SA183628MGH15Ames 27121
SA183629MGH103Ames 27124
SA183630MGH193Ames 27133
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Collection:

Collection ID:CO002018
Collection Summary:Entire shoots of 17-day old maize plants were collected and immediately frozen in liquid nitrogen. All samples were collected within one hour in the middle of the day (12:00-1:00 pm).
Sample Type:Plant
Collection Method:Cut entire shoots from 1 cm above ground and collected into 50 ml screw cap tube and quickly thrown into liquid N2.
Collection Location:University of Nebraska-Lincoln Innovation Campus greenhouse
Collection Frequency:once
Collection Duration:8/16/2018 12:00 pm - 1:00 pm
Volumeoramount Collected:Entire shoot of a single plant
Storage Conditions:-80℃
Collection Vials:50 ml falcon tube
Storage Vials:50 ml falcon tube
Collection Tube Temp:Liquid N2

Treatment:

Treatment ID:TR002037
Treatment Summary:Seeds were surface-sterilized, imbibed, and sown on Fafard germination soil. Plants were and cultured in a greenhouse with a temperature of 18-25 °C and a 14/10 h light/dark cycle for 14 days. All inbred lines were treated equally.
Plant Growth Support:Fafard germination soil
Plant Growth Location:University of Nebraska-Lincoln Innovation campus greenhouse
Plant Plot Design:Randomized
Plant Light Period:14/10 h light/dark cycle
Plant Temp:18-25 °C
Plant Watering Regime:daily
Plant Nutritional Regime:no nutrient addition during the experimental period
Plant Estab Date:2018-07-31
Plant Harvest Date:2018-08-13
Plant Growth Stage:Juvenile
Plant Metab Quench Method:Quick freezing by liquid nitrogen
Plant Harvest Method:cutting

Sample Preparation:

Sampleprep ID:SP002031
Sampleprep Summary:Whole samples were ground into fine powder under liquid nitrogen temperature and aliquoted 25 mg for metabolite analysis. The metabolites were extracted by methanol: water: chloroform and 50 µl of the upper aqueous phase was dried down to be derivatized by methoxyamination and trimethylsilylation.
Processing Storage Conditions:Room temperature
Extract Storage:Room temperature
Sample Resuspension:pyridine
Sample Derivatization:methoxyamination and trimethylsilylation
Sample Spiking:8.2 ug/ml ribitol

Combined analysis:

Analysis ID AN003169
Analysis type MS
Chromatography type GC
Chromatography system Agilent 7890B
Column Agilent HP5-MS (30m x 0.25mm, 0.25 um)
MS Type EI
MS instrument type GC-TOF
MS instrument name Agilent 7200 Q-TOF
Ion Mode POSITIVE
Units arbitrary unit (relative contents)

Chromatography:

Chromatography ID:CH002342
Instrument Name:Agilent 7890B
Column Name:Agilent HP5-MS (30m x 0.25mm, 0.25 um)
Column Temperature:80
Flow Gradient:None
Flow Rate:1 ml/min
Injection Temperature:230
Time Program:80 °C 2 min, ramp up to 330°C at 15 °C/min, hold for 6 min
Transferline Temperature:280℃
Sample Syringe Size:10 µl
Randomization Order:manual
Chromatography Type:GC

MS:

MS ID:MS002947
Analysis ID:AN003169
Instrument Name:Agilent 7200 Q-TOF
Instrument Type:GC-TOF
MS Type:EI
MS Comments:MassHunter software suits
Ion Mode:POSITIVE
Ion Source Temperature:250
Ionization:EI
Ionization Energy:70 eV
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