Summary of Study ST001961

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001248. The data can be accessed directly via it's Project DOI: 10.21228/M8D41C This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001961
Study TitleMetabolomics of brown adipose tissue in murine heart failure model
Study Typeuntargeted CE-TOF/MS metabolomic profiling
Study SummaryBrown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the changes of metabolites, we conducted the CE-TOF/MS analysis using BAT from TAC (thoracic aortic constriction) or sham-operated mice. In BAT from TAC-operated mice, we found increase of choline and glycerophosphorylcholine and a decrease of phosphorylcholine, suggesting that BAT dysfunction induces the disorientation of choline metabolism.
Institute
Juntendo University
DepartmentDepartment of Cardiovascular Biology and Medicine
Last NameYoshida
First NameYohko
Address2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan
Emailyohko105@yahoo.co.jp
Phone+81-3-3813-3111
Submit Date2021-10-13
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailMS(Dir. Inf.)
Release Date2022-11-01
Release Version1
Yohko Yoshida Yohko Yoshida
https://dx.doi.org/10.21228/M8D41C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR001248
Project DOI:doi: 10.21228/M8D41C
Project Title:Metabolomics of brown adipose tissue in murine heart failure model
Project Type:untargeted CE-TOF/MS metabolomic profiling
Project Summary:Brown adipose tissue (BAT) was initially characterised as a thermogenic organ, and recent studies have suggested it plays a crucial role in maintaining systemic metabolic health. In this project, we demonstrated that alteration of BAT function contributes to development of heart failure through disorientation in choline metabolism. To analyze the changes of metabolites, we conducted the CE-TOF/MS analysis using BAT from TAC (thoracic aortic constriction) or sham-operated mice. In BAT from TAC-operated mice, we found increase of choline and glycerophosphorylcholine and a decrease of phosphorylcholine, suggesting that BAT dysfunction induces the disorientation of choline metabolism.
Institute:Juntendo University
Department:Department of Cardiovascular Biology and Medicine
Last Name:Yoshida
First Name:Yohko
Address:2-1-1, Hongo, Bunkyo-ku, Tokyo, Tokyo, 1138421, Japan
Email:yohko105@yahoo.co.jp
Phone:+81-3-3813-3111

Subject:

Subject ID:SU002041
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Gender:Male

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Group
SA184620--
SA1846214BSham-operated
SA1846226BSham-operated
SA1846233BSham-operated
SA1846241BSham-operated
SA1846252BSham-operated
SA18462612BTAC-operated
SA18462711BTAC-operated
SA1846289BTAC-operated
SA1846297BTAC-operated
SA18463010BTAC-operated
Showing results 1 to 11 of 11

Collection:

Collection ID:CO002034
Collection Summary:Brown adipose tissue (BAT) was excised from the mice and immediately frozen in liquid nitrogen.
Sample Type:Adipose tissue

Treatment:

Treatment ID:TR002053
Treatment Summary:All mice were randomly allocated for surgical procedures under blinded condition. Thoracic aortic constriction (TAC) was performed in 11-week-old male mice, and sample was collected 2 weeks after surgery. Sham-operated mice underwent the same procedures, except for aortic constriction.

Sample Preparation:

Sampleprep ID:SP002047
Sampleprep Summary:To extract metabolites, 40~50mg snap-frozen samples were completely homogenized by Shake Master NEO (Bio Medical Science) in 500uL of methanol containing L-methionine sulfone (Wako 502-76641), methionine sulfone (MES, Dojindo 349-01623), and D-camphor-10-sulfonic acid sodium salt (CSA, Wako 037-01032) (all at 20 μM). After adding CHCl3 (500 μl) and distilled water (200 μl) with thorough mixing, centrifugation was performed at 4,600g for 15 min at 4°C. Then the aqueous layer (300 μl) was collected and transferred to a 5-kDa cutoff filter (UltrafreeMC-PLHCC; Human Metabolome Technologies, UFC3LCCNB-HMT). Centrifugation was performed at 9,100g for 3 hours at 20°C, after which the filtrate was centrifugally concentrated and dissolved in 50μl distilled water containing reference compounds (200 μM each of 3-aminopyrrolidine and trimesate).

Combined analysis:

Analysis ID AN003196 AN003197
Analysis type MS MS
Chromatography type None (Direct infusion) None (Direct infusion)
Chromatography system none none
Column none none
MS Type ESI ESI
MS instrument type Single quadrupole Single quadrupole
MS instrument name Agilent 6224 TOF Agilent 6224 TOF
Ion Mode NEGATIVE POSITIVE
Units nmol/g nmol/g

Chromatography:

Chromatography ID:CH002364
Instrument Name:none
Column Name:none
Chromatography Type:None (Direct infusion)

MS:

MS ID:MS002974
Analysis ID:AN003196
Instrument Name:Agilent 6224 TOF
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([13C isotopic ion of deprotonated acetic acid dimer (2CH3COOH - H)]-, m/z 120.03841), and ([Hexakis + deprotonated acetic acid (CH3COOH - H)]-, m/z 680.03554). Exact mass data were acquired at a rate of 1.5 spectra/s over a 50-1000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites.
Ion Mode:NEGATIVE
  
MS ID:MS002975
Analysis ID:AN003197
Instrument Name:Agilent 6224 TOF
Instrument Type:Single quadrupole
MS Type:ESI
MS Comments:Automatic recalibration of each acquired spectrum was performed using reference masses of reference standards ([2MeOH13Cisotope]-, m/z 66.06306), and ([Hexakis(2,2-difluoroethoxy)phosphazene]-, m/z 622.028963). Exact mass data were acquired at the rate of 1.5 cycles/s over a 50 to 1,000 m/z range. Raw CE-TOFMS data were processed using Master Hands ver. 2.17.0.8 software for the quantification of metabolites.
Ion Mode:POSITIVE
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