Summary of Study ST002023

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001285. The data can be accessed directly via it's Project DOI: 10.21228/M8MD8N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002023
Study TitleA targeted metabolomics study for assessing rodent thyroid toxicity
Study SummaryThe thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.
Institute
Helmholtz Centre for Environmental Research
DepartmentDepartment of Molecular Systems biology
LaboratoryFunctional Metabolomics
Last NameWang
First NameZhipeng
AddressPermoserstraße 15, Leipzig, Saxony, 04318, Germany
Emailzhipeng.wang@ufz.de
Phone+49 341 235 1078
Submit Date2021-12-20
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-12-20
Release Version1
Zhipeng Wang Zhipeng Wang
https://dx.doi.org/10.21228/M8MD8N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001285
Project DOI:doi: 10.21228/M8MD8N
Project Title:A targeted metabolomics study for assessing rodent thyroid toxicity
Project Summary:The thyroid gland regulates various physiological mechanisms in mammals/humans, such as individual development, cell proliferation and differentiation. Thus, disorders can lead to diseases. In this study, we aimed to apply targeted metabolomics approach to investigate rodent thyroid toxicity. For this purpose, male Wistar rats have been exposed to a direct (6-propyl-2-thiouracil, PTU) and an indirect (phenytoin) thyroid toxicant, respectively. Thereby, two doses (low:5ppm for PTU and 300ppm for phenytoin , high: 50ppm for PTU and 2400ppm for phenytoin) and three exposure time phase (short: 2 weeks, long:4 weeks, and long+recovery: 4weeks+2weeks) were investigated, allowing insights into the modes of action during thyroid toxicity. Targeted metabolomics were applied to both liver and thyroid gland tissue.
Institute:Helmholtz Centre for Environmental Research GmbH
Department:Department of Molecular Systems biology
Laboratory:Functional Metabolomics
Last Name:Wang
First Name:Zhipeng
Address:Permoserstraße 15, Leipzig, Saxony, 04318, Germany
Email:zhipeng.wang@ufz.de
Phone:+49 341 235 1078

Subject:

Subject ID:SU002105
Subject Type:Mammal
Subject Species:Rattus norvegicus
Taxonomy ID:10116

Factors:

Subject type: Mammal; Subject species: Rattus norvegicus (Factor headings shown in green)

mb_sample_id local_sample_id Short sample name Organ Treatment Concentration Period Recovery
SA189446L-A103-G11-ML103 Liver Control - 4 weeks 2 weeks
SA189447L-A104-G11-ML104 Liver Control - 4 weeks 2 weeks
SA189448L-A105-G11-ML105 Liver Control - 4 weeks 2 weeks
SA189449L-A108-G11-ML108 Liver Control - 4 weeks 2 weeks
SA189450L-A110-G11-ML110 Liver Control - 4 weeks 2 weeks
SA189451L-A112-G12-ML112 Liver Phenytoin 300 ppm 4 weeks 2 weeks
SA189452L-A114-G12-ML114 Liver Phenytoin 300 ppm 4 weeks 2 weeks
SA189453L-A116-G12-ML116 Liver Phenytoin 300 ppm 4 weeks 2 weeks
SA189454L-A117-G12-ML117 Liver Phenytoin 300 ppm 4 weeks 2 weeks
SA189455L-A118-G12-ML118 Liver Phenytoin 300 ppm 4 weeks 2 weeks
SA189456L-A122-G13-ML122 Liver Phenytoin 2400 ppm 4 weeks 2 weeks
SA189457L-A123-G13-ML123 Liver Phenytoin 2400 ppm 4 weeks 2 weeks
SA189458L-A124-G13-ML124 Liver Phenytoin 2400 ppm 4 weeks 2 weeks
SA189459L-A127-G13-ML127 Liver Phenytoin 2400 ppm 4 weeks 2 weeks
SA189460L-A128-G13-ML128 Liver Phenytoin 2400 ppm 4 weeks 2 weeks
SA189461L-A12-G2-ML12 Liver Phenytoin 300 ppm 2 weeks 0 weeks
SA189462L-A132-G14-ML132 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189463L-A135-G14-ML135 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189464L-A136-G14-ML136 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189465L-A139-G14-ML139 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189466L-A140-G14-ML140 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189467L-A142-G15-ML142 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189468L-A143-G15-ML143 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189469L-A144-G15-ML144 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189470L-A145-G15-ML145 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189471L-A148-G15-ML148 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189472L-A15-G2-ML15 Liver Phenytoin 300 ppm 2 weeks 0 weeks
SA189473L-A16-G2-ML16 Liver Phenytoin 300 ppm 2 weeks 0 weeks
SA189474L-A17-G2-ML17 Liver Phenytoin 300 ppm 2 weeks 0 weeks
SA189475L-A18-G2-ML18 Liver Phenytoin 300 ppm 2 weeks 0 weeks
SA189476L-A22-G3-ML22 Liver Phenytoin 2400 ppm 2 weeks 0 weeks
SA189477L-A23-G3-ML23 Liver Phenytoin 2400 ppm 2 weeks 0 weeks
SA189478L-A25-G3-ML25 Liver Phenytoin 2400 ppm 2 weeks 0 weeks
SA189479L-A28-G3-ML28 Liver Phenytoin 2400 ppm 2 weeks 0 weeks
SA189480L-A2-G1-ML2 Liver Control - 2 weeks 0 weeks
SA189481L-A30-G3-ML30 Liver Phenytoin 2400 ppm 2 weeks 0 weeks
SA189482L-A32-G4-ML32 Liver 6-propyl-2-thiouracil 5 ppm 2 weeks 0 weeks
SA189483L-A33-G4-ML33 Liver 6-propyl-2-thiouracil 5 ppm 2 weeks 0 weeks
SA189484L-A34-G4-ML34 Liver 6-propyl-2-thiouracil 5 ppm 2 weeks 0 weeks
SA189485L-A36-G4-ML36 Liver 6-propyl-2-thiouracil 5 ppm 2 weeks 0 weeks
SA189486L-A38-G4-ML38 Liver 6-propyl-2-thiouracil 5 ppm 2 weeks 0 weeks
SA189487L-A43-G5-ML43 Liver 6-propyl-2-thiouracil 50 ppm 2 weeks 0 weeks
SA189488L-A45-G5-ML45 Liver 6-propyl-2-thiouracil 50 ppm 2 weeks 0 weeks
SA189489L-A47-G5-ML47 Liver 6-propyl-2-thiouracil 50 ppm 2 weeks 0 weeks
SA189490L-A48-G5-ML48 Liver 6-propyl-2-thiouracil 50 ppm 2 weeks 0 weeks
SA189491L-A49-G5-ML49 Liver 6-propyl-2-thiouracil 50 ppm 2 weeks 0 weeks
SA189492L-A4-G1-ML4 Liver Control - 2 weeks 0 weeks
SA189493L-A52-G6-ML52 Liver Control - 4 weeks 0 weeks
SA189494L-A53-G6-ML53 Liver Control - 4 weeks 0 weeks
SA189495L-A55-G6-ML55 Liver Control - 4 weeks 0 weeks
SA189496L-A56-G6-ML56 Liver Control - 4 weeks 0 weeks
SA189497L-A58-G6-ML58 Liver Control - 4 weeks 0 weeks
SA189498L-A5-G1-ML5 Liver Control - 2 weeks 0 weeks
SA189499L-A62-G7-ML62 Liver Phenytoin 300 ppm 4 weeks 0 weeks
SA189500L-A65-G7-ML65 Liver Phenytoin 300 ppm 4 weeks 0 weeks
SA189501L-A66-G7-ML66 Liver Phenytoin 300 ppm 4 weeks 0 weeks
SA189502L-A67-G7-ML67 Liver Phenytoin 300 ppm 4 weeks 0 weeks
SA189503L-A6-G1-ML6 Liver Control - 2 weeks 0 weeks
SA189504L-A70-G7-ML70 Liver Phenytoin 300 ppm 4 weeks 0 weeks
SA189505L-A72-G8-ML72 Liver Phenytoin 2400 ppm 4 weeks 0 weeks
SA189506L-A73-G8-ML73 Liver Phenytoin 2400 ppm 4 weeks 0 weeks
SA189507L-A74-G8-ML74 Liver Phenytoin 2400 ppm 4 weeks 0 weeks
SA189508L-A76-G8-ML76 Liver Phenytoin 2400 ppm 4 weeks 0 weeks
SA189509L-A80-G8-ML80 Liver Phenytoin 2400 ppm 4 weeks 0 weeks
SA189510L-A82-G9-ML82 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 0 weeks
SA189511L-A85-G9-ML85 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 0 weeks
SA189512L-A88-G9-ML88 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 0 weeks
SA189513L-A89-G9-ML89 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 0 weeks
SA189514L-A8-G1-ML8 Liver Control - 2 weeks 0 weeks
SA189515L-A90-G9-ML90 Liver 6-propyl-2-thiouracil 5 ppm 4 weeks 0 weeks
SA189516L-A92-G10-ML92 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 0 weeks
SA189517L-A93-G10-ML93 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 0 weeks
SA189518L-A94-G10-ML94 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 0 weeks
SA189519L-A96-G10-ML96 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 0 weeks
SA189520L-A98-G10-ML98 Liver 6-propyl-2-thiouracil 50 ppm 4 weeks 0 weeks
SA189521S-A103-G11-MS103 Thyroid Control - 4 weeks 2 weeks
SA189522S-A104-G11-MS104 Thyroid Control - 4 weeks 2 weeks
SA189523S-A105-G11-MS105 Thyroid Control - 4 weeks 2 weeks
SA189524S-A108-G11-MS108 Thyroid Control - 4 weeks 2 weeks
SA189525S-A110-G11-MS110 Thyroid Control - 4 weeks 2 weeks
SA189526S-A112-G12-MS112 Thyroid Phenytoin 300 ppm 4 weeks 2 weeks
SA189527S-A114-G12-MS114 Thyroid Phenytoin 300 ppm 4 weeks 2 weeks
SA189528S-A116-G12-MS116 Thyroid Phenytoin 300 ppm 4 weeks 2 weeks
SA189529S-A117-G12-MS117 Thyroid Phenytoin 300 ppm 4 weeks 2 weeks
SA189530S-A118-G12-MS118 Thyroid Phenytoin 300 ppm 4 weeks 2 weeks
SA189531S-A122-G13-MS122 Thyroid Phenytoin 2400 ppm 4 weeks 2 weeks
SA189532S-A123-G13-MS123 Thyroid Phenytoin 2400 ppm 4 weeks 2 weeks
SA189533S-A124-G13-MS124 Thyroid Phenytoin 2400 ppm 4 weeks 2 weeks
SA189534S-A127-G13-MS127 Thyroid Phenytoin 2400 ppm 4 weeks 2 weeks
SA189535S-A128-G13-MS128 Thyroid Phenytoin 2400 ppm 4 weeks 2 weeks
SA189536S-A12-G2-MS12 Thyroid Phenytoin 300 ppm 2 weeks 0 weeks
SA189537S-A132-G14-MS132 Thyroid 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189538S-A135-G14-MS135 Thyroid 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189539S-A136-G14-MS136 Thyroid 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189540S-A139-G14-MS139 Thyroid 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189541S-A140-G14-MS140 Thyroid 6-propyl-2-thiouracil 5 ppm 4 weeks 2 weeks
SA189542S-A142-G15-MS142 Thyroid 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189543S-A143-G15-MS143 Thyroid 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189544S-A144-G15-MS144 Thyroid 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
SA189545S-A145-G15-MS145 Thyroid 6-propyl-2-thiouracil 50 ppm 4 weeks 2 weeks
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Collection:

Collection ID:CO002098
Collection Summary:The animal experiment was done by our cooperative partner BASF. The liver and thyroid tissue samples were delivered to us with the protection of dry ice. And all samples was stored immediately under -80℃ until further analysis.
Sample Type:Tissues

Treatment:

Treatment ID:TR002117
Treatment Summary:Two well-described test chemicals, i.e. Phenytoin and Propylthiouracil, were administered via the diet at each two concentrations to male Wistar rats for 2 and 4 weeks. In addition, a recovery period of 2 weeks without test substance application was included in order to monitor reversibility of the induced effects. Phenytoin was administered concentrations of 300 and 2400 ppm, Propylthiouracil was administered at concentrations of 5 and 50 ppm. Control animals received maintenance diet only without inclusion of any test substance.

Sample Preparation:

Sampleprep ID:SP002111
Sampleprep Summary:Tissue metabolites were extracted according to our laboratory standard protocol. Briefly, about 100 mg liver and all received thyroid gland (2.4 - 34.1 mg) tissue materials were weighed and mixed with 5x extraction buffer (ACN / H2O: 1:1). With the help of added 4 metal balls, the tissue metabolites were separated by using a tissue slicer for 10 min at 30 Hz. After centrifugation of 2 min at 14,000 rpm, the metabolites containing supernatant were transfered to a fresh tube, and stored at -80℃ for further analysis. The targeted metabolomics was done by using MxP® Quant 500 kit (Biocrates). All analysis steps was followed by the manufactures from Biocrates.

Combined analysis:

Analysis ID AN003293
Analysis type MS
Chromatography type Unspecified
Chromatography system Agilent 1290 Infinity
Column MxP Quant 500 System
MS Type ESI
MS instrument type QTRAP
MS instrument name ABI Sciex 5500 QTrap
Ion Mode UNSPECIFIED
Units µM

Chromatography:

Chromatography ID:CH002432
Instrument Name:Agilent 1290 Infinity
Column Name:MxP Quant 500 System
Chromatography Type:Unspecified

MS:

MS ID:MS003063
Analysis ID:AN003293
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:QTRAP
MS Type:ESI
MS Comments:Both FIA and LC methods were used. Both positive and negative were used. The MS acquisition and Data processing were done following the manufacturer's instructions. from Biocrates
Ion Mode:UNSPECIFIED
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