Summary of Study ST002149

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001362. The data can be accessed directly via it's Project DOI: 10.21228/M8NX37 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002149
Study TitleIn vivo commensal control of Clostridioides difficile virulence
Study TypeGnotobiotic mouse infection model
Study SummaryGnotobiotic mouse infection model with C. difficile strain ATCC43255 and co-colonization studies with the commensals Paraclostridium bifermentans or Clostridium sardiniense
Institute
Brigham and Women's Hospital
DepartmentPathology/Massachusetts Host-Microbiome Center
LaboratoryBry
Last NameBry
First NameLynn
Address221 Longwood Ave
Emaillbry@bwh.harvard.edu
Phone6177325500
Submit Date2021-12-09
Num Groups6
Total Subjects48
Num Males24
Num Females24
Publicationsdoi: 10.1016/j.chom.2021.09.007
Analysis Type DetailLC-MS
Release Date2022-05-13
Release Version1
Lynn Bry Lynn Bry
https://dx.doi.org/10.21228/M8NX37
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR001362
Project DOI:doi: 10.21228/M8NX37
Project Title:In vivo commensal control of Clostrioides difficile virulence
Project Type:Animal model
Project Summary:Untargeted metabolomic data (Metabolon) of cecal contents from gnotobiotic (GF) mice, GF mice monocolonized for 7 days with the commensals Paraclostridium bifermentans or Clostridium sardiniens, or for 24 hours with C. difficile strain ATCC43255 or co-colonized with one of the above commensals.
Institute:Brigham and Women's Hospital
Department:Pathology/Massachusetts Host-Microbiome Center
Laboratory:Bry
Last Name:Bry
First Name:Lynn
Address:221 Longwood Ave
Email:lbry@bwh.harvard.edu
Phone:6177325500
Funding Source:BWH Precision Medicine Institute; NIH: R01 AI153605, P30DK034854, R01AI128215, R01AI141953, U19AI135976 T32 HL007627; NSF: IIBR-2042948; Pasteur Bourse ROUX
Publications:doi:10.1016/j.chom.2021.09.007 doi:10.1016/j.chom.2021.09.008

Subject:

Subject ID:SU002235
Subject Type:Mammal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:Clostridioides difficile ATCC43255
Age Or Age Range:6 weeks
Gender:Male and female
Animal Animal Supplier:Massachusetts Host-Microbiome Center Gnotobiotic Resource
Animal Housing:Germfree
Animal Water:sterile, ad libitum

Factors:

Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA205906Cdiff4C. difficile monocolonized
SA205907Cdiff3C. difficile monocolonized
SA205908Cdiff5C. difficile monocolonized
SA205909Cdiff7C. difficile monocolonized
SA205910Cdiff8C. difficile monocolonized
SA205911Cdiff2C. difficile monocolonized
SA205912Cdiff6C. difficile monocolonized
SA205913Cdiff1C. difficile monocolonized
SA205914CSAR/Cdiff5C. sardiniense + C. difficile co-colonized
SA205915CSAR/Cdiff6C. sardiniense + C. difficile co-colonized
SA205916CSAR/Cdiff4C. sardiniense + C. difficile co-colonized
SA205917CSAR/Cdiff3C. sardiniense + C. difficile co-colonized
SA205918CSAR/Cdiff1C. sardiniense + C. difficile co-colonized
SA205919CSAR/Cdiff2C. sardiniense + C. difficile co-colonized
SA205920CSAR/Cdiff8C. sardiniense + C. difficile co-colonized
SA205921CSAR/Cdiff7C. sardiniense + C. difficile co-colonized
SA205922CSAR3C. sardiniense monocolonized
SA205923CSAR1C. sardiniense monocolonized
SA205924CSAR2C. sardiniense monocolonized
SA205925CSAR4C. sardiniense monocolonized
SA205926CSAR5C. sardiniense monocolonized
SA205927CSAR7C. sardiniense monocolonized
SA205928CSAR8C. sardiniense monocolonized
SA205929CSAR6C. sardiniense monocolonized
SA205930GF5Germfree
SA205931GF4Germfree
SA205932GF3Germfree
SA205933GF2Germfree
SA205934GF6Germfree
SA205935GF1Germfree
SA205936GF8Germfree
SA205937GF7Germfree
SA205938PBI/Cdiff4P. bifermentans + C. difficile co-colonized
SA205939PBI/Cdiff2P. bifermentans + C. difficile co-colonized
SA205940PBI/Cdiff5P. bifermentans + C. difficile co-colonized
SA205941PBI/Cdiff1P. bifermentans + C. difficile co-colonized
SA205942PBI/Cdiff7P. bifermentans + C. difficile co-colonized
SA205943PBI/Cdiff8P. bifermentans + C. difficile co-colonized
SA205944PBI/Cdiff3P. bifermentans + C. difficile co-colonized
SA205945PBI/Cdiff6P. bifermentans + C. difficile co-colonized
SA205946PBI1P. bifermentans monocolonized
SA205947PBI2P. bifermentans monocolonized
SA205948PBI7P. bifermentans monocolonized
SA205949PBI8P. bifermentans monocolonized
SA205950PBI6P. bifermentans monocolonized
SA205951PBI5P. bifermentans monocolonized
SA205952PBI4P. bifermentans monocolonized
SA205953PBI3P. bifermentans monocolonized
Showing results 1 to 48 of 48

Collection:

Collection ID:CO002228
Collection Summary:Cecal contents
Sample Type:Cecum
Collection Method:Snap freeze in cryovials on liquid nitrogen
Storage Conditions:-80℃
Additives:None

Treatment:

Treatment ID:TR002247
Treatment Summary:Gnotobiotic mice (baseline controls) or mono- or co-colonized with C. difficile, and the commensal P. bifermentans or C. sardiniense.
Treatment Dose:gavage with 1000 spores of ATCC43255 for C. difficile infection; 200uL of overnight culture for commensal species

Sample Preparation:

Sampleprep ID:SP002241
Sampleprep Summary:Cecum was dissected from mice and contents immediately transferred into cryovials for snap freezing on liquid nitrogen. Materials stored at -80*C until shipment to Metabolon, on dry ice, for analyses.
Processing Storage Conditions:-80℃
Extract Storage:-80℃

Combined analysis:

Analysis ID AN003519
Analysis type MS
Chromatography type Unspecified
Chromatography system Unspecified
Column Unspecified
MS Type Unspecified
MS instrument type Unspecified
MS instrument name Metabolon
Ion Mode UNSPECIFIED
Units OriginalScale MS Units

Chromatography:

Chromatography ID:CH002598
Chromatography Summary:None
Instrument Name:Unspecified
Column Name:Unspecified
Chromatography Type:Unspecified

MS:

MS ID:MS003277
Analysis ID:AN003519
Instrument Name:Metabolon
Instrument Type:Unspecified
MS Type:Unspecified
MS Comments:Metabolon global untargeted screen of cecal contents
Ion Mode:UNSPECIFIED
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