Summary of Study ST003425
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002117. The data can be accessed directly via it's Project DOI: 10.21228/M8ZV6G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST003425 |
Study Title | Targeted free fatty acids metabolomics studies on serum, cecal content, cecum tissue, chow diet, and Tritrichomonas mu cells |
Study Summary | Serum, cecal content, cecum tissue samples of murine, chow diet, and T.mu cells were collected to perform the targeted free fatty acids metabolome analysis. The aim of this study was to verify that whether T.mu could release free PUFA (especially ARA) to the intestinal tract of its host by comparing the PUFA concentration in the freshly isolated T.mu cells, chow diet, cecal content and serum of the host (Mus musculus).Based on the result of this targeted free fatty acids metabolomics studies, we found that T.mu could release ARA to the intestinal tract of its host and increase the concentration of ARA in its host's intestinal tract. |
Institute | Xuzhou medical university |
Last Name | Kou |
First Name | Yanbo |
Address | Tongshan road 209, Xuzhou, Jiangsu, 221004, China |
fightingkyb@163.com | |
Phone | +86-051683262123 |
Submit Date | 2024-08-20 |
Raw Data Available | Yes |
Raw Data File Type(s) | cdf |
Analysis Type Detail | GC-MS |
Release Date | 2024-09-10 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
Project ID: | PR002117 |
Project DOI: | doi: 10.21228/M8ZV6G |
Project Title: | A mouse protozoan boosts antigen-specific mucosal IgA responses in a specific lipid metabolism- and signaling-dependent manner |
Project Summary: | IgA antibodies play an important role in mucosal immunity. However, there is still no effective way to consistently boost mucosal IgA responses, and the factors influencing these responses are not fully understood. We observed that colonization with the murine intestinal symbiotic protozoan Tritrichomonas musculis (T.mu) boosted antigen-specific mucosal IgA responses in wild-type C57BL/6 mice. This enhancement was attributed to the accumulation of free arachidonic acid (ARA) in the intestinal lumen, which served as a signal to stimulate the production of antigen-specific mucosal IgA. When ARA was prevented from undergoing its downstream metabolic transformation using the 5-lipoxygenase inhibitor zileuton or by blocking its downstream biological signaling through genetic deletion of the Leukotriene B4 receptor 1 (Blt1), the T.mu-mediated enhancement of antigen-specific mucosal IgA production was suppressed. Moreover, both T.mu transfer and dietary supplementation of ARA augmented the efficacy of an oral vaccine against Salmonella infection, with this effect being dependent on Blt1. Our findings elucidate a tripartite circuit linking nutrients from the diet or intestinal microbiota, host lipid metabolism, and the mucosal humoral immune response. |
Institute: | Xuzhou medical university |
Last Name: | Kou |
First Name: | Yanbo |
Address: | Tongshan road 209, Xuzhou, Jiangsu, 221004, China |
Email: | fightingkyb@163.com |
Phone: | +86-051683262123 |
Subject:
Subject ID: | SU003552 |
Subject Type: | Mammal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Gender: | Not applicable |
Species Group: | Mammals |
Factors:
Subject type: Mammal; Subject species: Mus musculus (Factor headings shown in green)
mb_sample_id | local_sample_id | Sample source | Treatment |
---|---|---|---|
SA377682 | T_mu_content | cecal content | colonized with Tmu |
SA377683 | Ctrl_content | cecal content | control |
SA377684 | T_mu_cecum | cecum tissue | colonized with Tmu |
SA377685 | Ctrl_cecum | cecum tissue | control |
SA377686 | Diet | chow diet | ground to power |
SA377687 | T_mu_serum | serum | colonized with Tmu |
SA377688 | Ctrl_serum | serum | control |
SA377681 | T_mu | T_mu cells | isolated from cecal content |
Showing results 1 to 8 of 8 |
Collection:
Collection ID: | CO003545 |
Collection Summary: | WT C57/BL6 mice were colonized with or without T.mu for 7 days, and the serum, cecum tissue, cecal content samples were collected on day 7. For T.mu cells sample, T.mu cells were isolated and counted from T.mu positive cecal content. Samples were washed with cold PBS and then flash-frozen in liquid nitrogen. |
Sample Type: | Serum, cecum tissue, cecal content, Tritrichomonas musculis cells, chow diet |
Treatment:
Treatment ID: | TR003561 |
Treatment Summary: | WT C57/BL6 mice were colonized with or without T.mu for 7 days, and the serum, cecum tissue, cecal content samples were collected on day 7. For T.mu cells sample, T.mu cells were isolated and counted from T.mu positive cecal content. For diet sample, the chow diet power was used. |
Sample Preparation:
Sampleprep ID: | SP003559 |
Sampleprep Summary: | The weighed cecal content, cecum tissue, serum, animal chow diet powder, or enumerated T.mu cells were transferred into new 2 mL EP tubes followed extraction with 500 μL extracting solution [Isopropanol : n-Hexane = 2:3 (V:V)], including 0.2 mg/L internal standard). After homogenized in a ball mill for 4 min at 40 Hz and a 5 min ultrasound treatment, the samples were centrifuged 16200 × g for 15 min at 4 °C. Then the supernatants were transferred into new 2 mL EP tubes followed with nitrogen blow dry. The dried samples were resuspended in 500 μL of methanol: trimethylsilyl diazomethane solution (1:2), after standing 30 min at room temperature, another nitrogen blow dry was performed. Then, the samples were resuspended in 160 μL of n-hexane followed with a centrifugation of 16200 × g for 1 min. Finally, the supernatants were transferred into new vials for GC-MS analysis. |
Combined analysis:
Analysis ID | AN005624 |
---|---|
Analysis type | MS |
Chromatography type | GC |
Chromatography system | Agilent 7890B |
Column | Agilent DB-FastFAME capillary column (90 m × 0.25 mm × 0.25 um) |
MS Type | EI |
MS instrument type | Single quadrupole |
MS instrument name | Agilent 5977B |
Ion Mode | POSITIVE |
Units | μg/ml for serum, μg/g for cecal content, μg/g for diet, μg/8*10^7 T.mu cells |
Chromatography:
Chromatography ID: | CH004274 |
Chromatography Summary: | DB-FastFAME capillary column (90 m × 0.25 mm × 0.25 µm, Agilent Technologies) |
Instrument Name: | Agilent 7890B |
Column Name: | Agilent DB-FastFAME capillary column (90 m × 0.25 mm × 0.25 um) |
Column Temperature: | 230 |
Flow Gradient: | - |
Flow Rate: | - |
Solvent A: | - |
Solvent B: | - |
Chromatography Type: | GC |
MS:
MS ID: | MS005348 |
Analysis ID: | AN005624 |
Instrument Name: | Agilent 5977B |
Instrument Type: | Single quadrupole |
MS Type: | EI |
MS Comments: | Mass spectrometry data were acquired in Scan/SIM mode over an m/z range of 33-400 after a solvent delay of 7 minutes. Fatty acid methyl esters (FAMEs) were identified by comparing them to commercial FAME standards (ANPEL, Shanghai, China, Cat# CDAA-252). The absolute concentrations of FAMEs were calculated using calibration curves based on the internal standard method. |
Ion Mode: | POSITIVE |