Summary of Study ST000335

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000267. The data can be accessed directly via it's Project DOI: 10.21228/M88K54 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000335
Study TitleMetabolomics of bovine uterine fluid at the onset of conceptus elongation
Study TypeProspective cohort study
Study SummaryThe objective is to investigate changes in metabolomics of uterine lumen content of lactating dairy cows associated with the onset of conceptus (embryo and associated membranes) elongation. Lactating dairy cows had estrous cycles synchronized and were subjected to induced ovulation and timed artificial insemination (AI). The day of AI was considered study d 0. On d 15, uteri were flushed by transcervical catheterization and infusion of 20 mL of phosphate buffered solution with 0.1% of polyvinyl acetate. Recovered conceptuses were classified based on morphology/length as ovoid (OV; 1-4 mm), tubular (TUB; 5-19 mm) and filamentous (FIL; 20-85 mm). The first 20 mL infused in the uterus were recovered, placed in conical tubes and centrifuged at 2,000 × g at 4ᵒC. The supernatant was collect, aliquoted and stored at -80ᵒC for later analyses of fluid composition, including measurement of IFN-τ concentration. Cows with no conceptus recovered and no detection of IFN-τ in uterine flushing were considered as nonpregnant (NPREG). The experimental design was then considered a prospective cohort study with 4 independent groups (NPREG, OV, TUB, and FIL). The additional 5th group represents a specific physiological condition of cows within the study and it will be compared to TUB and FIL groups combined, working as a pilot study for future research.
University of Florida
LaboratoryLaboratory of Reproduction and Nutrition of Dairy Cows
Last NameRibeiro
First NameEduardo
Address2250 Shealy Drive, Bldg. 499, Room 228, Gainesville, FL 32611-0910
Submit Date2016-01-17
Num Groups5
Total Subjects30
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2017-01-17
Release Version1
Eduardo Ribeiro Eduardo Ribeiro application/zip

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Combined analysis:

Analysis ID AN000540 AN000541
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000 Thermo Scientific-Dionex Ultimate 3000
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Units peak area peak area