Summary of Study ST000469
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000469 |
Study Title | Enterococcus faecalis nucleotide profiles - pilot |
Study Type | Comparison between wild-type and mutant strain, as well as providing varying cell count input for WT |
Study Summary | E. faecalis OG1RF and rel/relQ will be grown in FMC-AUG to OD600 = 0.25. 3E10 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation. This process will be repeated with OG1RF only to harvest 3E9 and 3E8 cells. A second OG1RF sample of 3E10 cells will be scraped into formic acid; only acid-soluble extract willl be sent for this sample. |
Institute | University of Florida |
Department | SECIM |
Laboratory | Lemos-Abranches |
Last Name | Kajfasz |
First Name | Jessica |
Address | 1395 Center Drive, PO Box 100424 Gainesville, FL 32610 |
jkajfasz@dental.ufl.edu | |
Phone | 352-273-6675 |
Submit Date | 2016-09-10 |
Num Groups | 5 |
Total Subjects | 5 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw |
Analysis Type Detail | LC-MS |
Release Date | 2017-10-03 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000732 | AN000733 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
Column | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | Triple quadrupole | Triple quadrupole |
MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ |
Ion Mode | POSITIVE | POSITIVE |
Units | nmol/mg protein | nmol/mg protein |