Summary of Study ST001512

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001019. The data can be accessed directly via it's Project DOI: 10.21228/M80408 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001512
Study TitleDiel investments in phytoplankton metabolite production influenced by associated heterotrophic bacteria
Study TypeIncubation experiment
Study SummaryOrganic substrate transfer between photoautotrophic and heterotrophic microbes in the surface ocean is a central but poorly understood process in the global carbon cycle. This study developed a co-culture system of marine diatom Thalassiosira pseudonana and heterotrophic bacterium Ruegeria pomeroyi, and addressed diel changes in phytoplankton endometabolite production using nuclear magnetic resonance (NMR) and bacterial metabolite consumption using gene expression. Here we deposit data for NMR analysis from the study. Samples were collected every 6 hours over two days under a diel light cycle. During the course of the study, we observed an increase in some phytoplankton endometabolites presumably due to the effects of the associated bacteria. We introduced an additional experiment and tested this possibility by comparing phytoplankton endometabolite accumulation between axenic treatments and bacteria coculture treatments.
Institute
University of Georgia
DepartmentDepartment of Marine Sciences; Complex Carbohydrate Research Center
LaboratoryMoran Lab; Edison Lab
Last NameUchimiya
First NameMario
Address315 Riverbend Rd, Athens, GA 30602
Emailmario.uchimiya@uga.edu
Phone‭(706) 542-8387‬
Submit Date2020-10-18
Raw Data AvailableYes
Analysis Type DetailNMR
Release Date2020-11-02
Release Version1
Mario Uchimiya Mario Uchimiya
https://dx.doi.org/10.21228/M80408
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Analysis:

Analysis ID:AN002506
Laboratory Name:Complex Carbohydrate Research Center
Analysis Type:NMR
Analysis Protocol File:5_Analysis protocol_UGA_phytoplankton_Oct2020.docx
Acquisition Parameters File:6_Acquisition and processing parameters_UGA_phytoplankton_Oct2020.xlsx
Software Version:TopSpin 3.5.7, 4.0.1, and 4.0.4
Operator Name:Mario Uchimiya
Processing Parameters File:6_Acquisition and processing parameters_UGA_phytoplankton_Oct2020.xlsx
Data Format:Bruker
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