Summary of Study ST001622

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001041. The data can be accessed directly via it's Project DOI: 10.21228/M84M6M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001622
Study TitleIsotope Tracing Analysis in Intestinal Organoids with fructose
Study SummaryWe isolated and cultured Intestinal Organoids from Mouse. To trace the metabolism of fructose, intestinal organoids were cultured in unlabeled fructose-containing DMEM/F12 medium (10 mM D-fructose, 0 mM D-glucose, 2.5 mM L-glutamine) for 12 hr, and then switched into 13C labeled fructose-containing medium (10 mM U-[13C] D-fructose, Sigma-aldrich) for 6 hr.
Institute
China Pharmaceutical University
DepartmentSchool of Medicine
LaboratoryMetabonomics
Last NameHou
First NameYuanlong
Addresstongjiaxiang, nanjing, jiangsu, 210000, China
Emailjian2103@163.com
Phone18851105337
Submit Date2020-12-09
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2020-12-16
Release Version1
Yuanlong Hou Yuanlong Hou
https://dx.doi.org/10.21228/M84M6M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002657
Analysis type MS
Chromatography type HILIC
Chromatography system UPLC-Q-TOF-MS (Synapt G2si, waters)
Column Waters XBridge Amide (100 x 4.6mm,3.5um)
MS Type ESI
MS instrument type GC-TOF
MS instrument name Waters Synapt G2 Si QTOF
Ion Mode NEGATIVE
Units abundance
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