Summary of Study ST001927
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001217. The data can be accessed directly via it's Project DOI: 10.21228/M8D693 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001927 |
| Study Title | Fungal consortium of two Beauveria bassiana strains increases their virulence, growth, and resistance to stress: a metabolomic approach. |
| Study Type | Untargeted Metabolomics |
| Study Summary | Entomopathogenic fungi have been successfully used to control agricultural pests. They infect insects by coming into direct contact with their cuticle or when feeding on contaminated leaves or fruits. After contact with the insect, the entomopathogenic fungus penetrates its body cavity, where it grows and colonizes it from within, causing its death The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. Despite recent developments and growing efforts to better understand fungal metabolism and metabolites, much remains unknown. Metabolomics therefore represents an important field for evaluating the metabolites produced or modified by an organism or its relationship with the environment. In the present study, we aim to use untargeted metabolomics with gas and liquid chromatography coupled to mass spectrometers (GC-MS and LC-MS/MS) to evaluate the metabolic alterations caused by the co-cultivation of these strains and to correlate the metabolites produced by this consortium with the increased mortality in D. fovealis observed previosly. |
| Institute | Universidade Federal do Paraná |
| Department | Patologia Básica |
| Laboratory | Laboratório de Microbiologia e Biologia Molecular |
| Last Name | Stuart |
| First Name | Andressa |
| Address | Av. Cel. Francisco Heráclito dos Santos, 100, 81530000, Jardim das Américas, Curitiba, Paraná, Brasil |
| andressa.katiski@gmail.com | |
| Phone | 5541991922779 |
| Submit Date | 2021-09-29 |
| Num Groups | 3 |
| Total Subjects | 15 |
| Study Comments | Two genetically distinct strains of B. bassiana (Bov 3 and Bov 2) were cultivated in Agar Sabouraud culture medium, both separately and co-cultivated to form a fungal consortium. The metabolomic analysis were performed at the Laboratório de Genética de Plantas Max Feffer facility of the Escola Superior de Agricultura Luiz de Queiroz of the Universidade de São Paulo (ESALQ/USP). Three reads of every biological replicate (five per treatment) were performed, generating fifteen readings for each treatment. Pools of metabolites from each group were created as a quality control. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, raw(Waters) |
| Analysis Type Detail | GC/LC-MS |
| Release Date | 2022-10-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN003133 | AN003134 | AN003135 |
|---|---|---|---|
| Analysis type | MS | MS | MS |
| Chromatography type | GC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 7890A | Waters Acquity UPLC | Waters Acquity UPLC |
| Column | Agilent DB-5 (20m x 0.18mm, 0.18um); Restek RX-T 17 (0.9m x 0.10mm, 0.10um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) | Waters Acquity UPLC HSS (100 x 2.1mm, 1.7um) |
| MS Type | EI | ESI | ESI |
| MS instrument type | GC x GC-TOF | QTOF | QTOF |
| MS instrument name | Leco Pegasus 4D GCxGC TOF | Waters Ultima QTOF | Waters Ultima QTOF |
| Ion Mode | UNSPECIFIED | POSITIVE | NEGATIVE |
| Units | Relative intensity | Relative intensity |
