Summary of Study ST002206

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001409. The data can be accessed directly via it's Project DOI: 10.21228/M8KT4Z This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002206
Study TitleLipolysis-derived Lipids Determine Autophagy Initiation during Fasting
Study SummaryFor survival, autophagy is a crucial intracellular self-degradation process to provide energy sources, helping adapt to nutrient deprivation. Although nutrient availability is a key determinant of autophagy initiation, it remains elusive underlying mechanism(s) of perceiving nutritional scarcity by which cells timely turn on autophagy as the last self-destructive process for energy supply. Here, we showed that PKA-dependent lipolysis can block the initiation of futile autophagy during short-term nutritional deprivation by repressing AMPK. Using Raman microscopy imaging and metabolomics, we found that autophagy occurred by reduction in available free fatty acids (FFAs) for energy sources. By modulating genes involved in lipolysis and fatty acid oxidation, we found that the use of lipolysis-derived FFAs precedes autophagy initiation. The dysregulated autophagy suppression during short-term fasting decreased motility and lifespan extension of worms. Taken together, these data suggest that PKA is a pivotal factor to orchestrate sophisticated catabolic pathways, preferring the use of PKA-mediated lipolytic products to repress futile autophagic degradation during short-term fasting through AMPK inhibition.
Institute
Seoul National University
Last NameJi
First NameYul
AddressSan 56-1, Sillim-Dong, Kwanak-Gu, Seoul, Seoul, 08826, Korea, South
Emailwithyul@snu.ac.kr
Phone01025076912
Submit Date2022-04-27
Analysis Type DetailLC-MS
Release Date2022-07-22
Release Version1
Yul Ji Yul Ji
https://dx.doi.org/10.21228/M8KT4Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN003609
Analysis type MS
Chromatography type Unspecified
Chromatography system Agilent 1200 RR Series II
Column ODS
MS Type ESI
MS instrument type LC/MSD
MS instrument name Agilent CE-TOFMS
Ion Mode POSITIVE
Units relative area
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