Summary of Study ST003340

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002071. The data can be accessed directly via it's Project DOI: 10.21228/M8X835 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003340
Study TitleEffect of feeding and the mTORC1 activity on metabolism in Caenorhabditis elegans
Study SummaryCellular growth and organismal development are remarkably complex processes that require the nutrient-responsive kinase mTORC1. Anticipating that important mTORC1 functions remained to be identified, we employed genetic and bioinformatic screening in C. elegans to uncover mechanisms of mTORC1 action. Here we show that during larval growth nutrients induce an extensive reprogramming of gene expression and alternative mRNA splicing by acting through mTORC1. mTORC1 regulates mRNA splicing and production of protein-coding mRNA isoforms largely independently of its target p70S6K, by increasing the activity of the serine/arginine-rich (SR) protein RSP-6 (SRSF3/7) and other splicing factors. mTORC1-mediated mRNA splicing regulation is critical for growth, mediates nutrient control of mechanisms that include energy, nucleotide, amino acid, and other metabolic pathways, and may be conserved in humans. Although mTORC1 inhibition delays aging, mTORC1-induced mRNA splicing promotes longevity, suggesting that when mTORC1 is inhibited enhancement of this splicing might provide additional anti-aging benefits.
Institute
Hiroshima University
Last NameOgawa
First NameTakafumi
Address1-3-1 Kagamiyama, Higashihiroshima, Hiroshima, 739-8530, Japan
Emailtakafumi-ogawa@hiroshima-u.ac.jp
Phone+81-80-9790-5278
Submit Date2024-07-17
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-11-20
Release Version1
Takafumi Ogawa Takafumi Ogawa
https://dx.doi.org/10.21228/M8X835
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN005474
Analysis type MS
Chromatography type HILIC
Chromatography system Thermo Vanquish
Column iHILIC®-(P) Classic HILIC Column PEEK (150 x 2.1mm, 5µm) Cat.# W 160.152.0520
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive HF-X Orbitrap
Ion Mode NEGATIVE
Units peak areas
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