Summary of Study ST003808
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002382. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ7Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003808 |
| Study Title | Glucose-6-phosphate-dehydrogenase on old peroxisomes maintains self-renewal of epithelial stem cells after asymmetric cell division |
| Study Summary | Peroxisomes play a crucial role in cellular metabolism. Glucose-6-phosphate dehydrogenase (G6PD), the gatekeeper enzyme of the pentose phosphate pathway, is primarily localized in the cytosol. However, studies have reported its presence in peroxisomes as well. This project aims to determine the function of G6PD on the peroxisomal membrane. In this study, we overexpressed G6PD in either the cytosol or on the peroxisomal membrane of mammary epithelial stem-like cells (hMECs). By comparing their lipidomic profiles, we found that peroxisomal membrane-associated G6PD provides NADPH, which feeds into peroxisomal ether lipid synthesis. |
| Institute | University of Helsinki |
| Department | Faculty of Biological and Environmental Sciences |
| Laboratory | Katajisto Laboratory |
| Last Name | Hien |
| First Name | Bui |
| Address | Biocenter 1, Viikinkaari 9 |
| hien.bui@helsinki.fi | |
| Phone | +358294159407 |
| Submit Date | 2025-03-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf, mzdata.xml |
| Analysis Type Detail | GC-MS/LC-MS |
| Release Date | 2025-03-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN006261 | AN006262 |
|---|---|---|
| Chromatography ID | CH004749 | CH004750 |
| MS ID | MS005963 | MS005964 |
| Analysis type | MS | MS |
| Chromatography type | GC | Reversed phase |
| Chromatography system | Shimadzu GC-2010 | Agilent 1290 Infinity |
| Column | Phenomenex Zebron ZB-wax capillary (30m x 0.25mm, 0.25um) | Phenomenex Luna Omega C18 (50 x 2.1mm, 1.6um, 100 Å) |
| MS Type | EI | ESI |
| MS instrument type | GC-FID & GC-MSD | Triple quadrupole |
| MS instrument name | Shimadzu QP2010 Plus | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | pmol/sample | peak area |
