Summary of Study ST000017
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000016. The data can be accessed directly via it's Project DOI: 10.21228/M86P45 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000017 |
| Study Title | Rat HCR/LCR Stamina Study |
| Study Type | LC-MS analysis (Untargeted) |
| Study Summary | Maximal exercise-associated oxidative capacity is strongly correlated with health and longevity in humans. Rats selectively bred for high running capacity (HCR) have improved metabolic health and are longer-lived than their low-capacity counterparts (LCR). Using metabolomic and proteomic profiling, we show that HCR efficiently oxidize fatty acids (FAs) and branched-chain amino acids (BCAAs), sparing glycogen and reducing accumulation of short- and medium-chain acylcarnitines. HCR mitochondria have reduced acetylation of mitochondrial proteins within oxidative pathways at rest, and there is rapid protein deacetylation with exercise, which is greater in HCR than LCR. Fluxomic analysis of valine degradation with exercise demonstrates a functional role of differential protein acetylation in HCR and LCR. Our data suggest that efficient FA and BCAA utilization contribute to high intrinsic exercise capacity and the health and longevity benefits associated with enhanced fitness. Research is published, untargeted data not used in publication but project description is relevant: http://www.sciencedirect.com/science/article/pii/S1550413115000595 |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Laboratory | Burant Lab (MMOC) |
| Last Name | Qi |
| First Name | Nathan |
| nathanqi@med.umich.edu | |
| Phone | 734-232-0815 |
| Submit Date | 2013-09-24 |
| Num Groups | 2 |
| Total Subjects | 42 |
| Raw Data Available | Yes |
| Uploaded File Size | 6.8 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2013-10-24 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000034 | AN000035 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1200 | Agilent 1200 |
| Column | Waters Acquity HSS T3 reversed-phase | Waters Acquity HSS T3 reversed-phase |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Agilent 6530 QTOF | Agilent 6530 QTOF |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000017 |
| Chromatography Summary: | Positive ion detection |
| Methods Filename: | EX00166-LCMS-method.pdf |
| Instrument Name: | Agilent 1200 |
| Column Name: | Waters Acquity HSS T3 reversed-phase |
| Column Temperature: | 40C |
| Flow Gradient: | 0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% methanol; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000018 |
| Chromatography Summary: | Negative ion detection |
| Methods Filename: | EX00166-LCMS-method.pdf |
| Instrument Name: | Agilent 1200 |
| Column Name: | Waters Acquity HSS T3 reversed-phase |
| Column Temperature: | 40C |
| Flow Gradient: | 0-0.5 min 1% B, 0.5-2 min 1-99% B, 2-6 min 99% B, 6-9 min 1% B. |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.1% ammonium bicarbonate |
| Solvent B: | 100% methanol; 0.1% ammonium bicarbonate |
| Chromatography Type: | Reversed phase |
