Summary of Study ST000095

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000087. The data can be accessed directly via it's Project DOI: 10.21228/M8F30Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000095
Study TitleDysfunctional lipid metabolism underlies the effect of perinatal DDT exposure on the development of metabolic syndrome
Study TypeChemical dosage and feeding study
Study SummaryTargeted metabolomic analysis of bile acids was performed on 15 mouse liver samples collected from mice euthanized at 9 months following consumption of a high fat diet w/o perinatal DDT exposure. Funded by the National Institute of Health (R00 ES019919, R03 DK082724, U24 DK092993, U24 DK097154, T32 ES007059, and P60 DK020541), the American Diabetes Association, and USDA-ARS intramural Project 5306-51530-019-00D. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization.
Institute
University of California, Davis
DepartmentU.S.D.A. Western Human Nutrition Research Center
LaboratoryNewman
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Emailjohn.newman@ars.usda.gov
Phone+1-530-752-1009
Submit Date2014-07-10
Num Groups2
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s)mzML
Uploaded File Size23 M
Analysis Type DetailLC-MS
Release Date2015-02-03
Release Version1
John Newman John Newman
https://dx.doi.org/10.21228/M8F30Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000151
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity UPLC
Column Waters Acquity BEH C18 (100 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name ABI Sciex API 6500 QTrap
Ion Mode NEGATIVE
Units % abundance

Chromatography:

Chromatography ID:CH000107
Chromatography Summary:Targeted UPLC-MS/MS method
Methods Filename:Newman_Lab_Bile_Acids_Extraction_&_Analysis_Protocol_La_Merrill_WCMC_P&F.pdf
Instrument Name:Waters Acquity UPLC
Column Name:Waters Acquity BEH C18 (100 x 2mm,1.7um)
Column Temperature:60 C
Flow Gradient:Yes
Flow Rate:0.4mL/min
Internal Standard:See protocol/methods file
Sample Injection:5 L
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:16 min
Weak Wash Solvent Name:20% methanol, 10% isopropanol
Weak Wash Volume:600 L
Strong Wash Solvent Name:50:50 Acetonitrile:Methanol
Strong Wash Volume:600 L
Sample Loop Size:17 L
Chromatography Type:Reversed phase
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