Summary of study ST000119

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000106. The data can be accessed directly via it's Project DOI: 10.21228/M8RC75 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000119
Study TitleMetabolomics analysis of multiple metabolic functions in the YjgF/YER057c/UK114 (Rid) protein family LCMS
Study Typewildtype vs knock-out
Study SummaryMetabolomics analysis of wild-type S. enterica, single RidA knock-out and triple Rid (ridA Rid2 Rid7) knock-out S.enterica cells
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryFiehn Laboratory
Last NameElBadawi-Sidhu
First NameMona
Address451 Health Sciences Drive, Davis, California 95616, USA
Emailmmelbadawi@ucdavis.edu
Submit Date2014-11-06
Num Groups4
Total Subjects6
Study CommentsSamples were measured in poitive and negative ionization mode. MS/MS files contain product ion scans (MS/MS) spectra for compound identification.
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2014-11-06
Release Version1
Mona ElBadawi-Sidhu Mona ElBadawi-Sidhu
https://dx.doi.org/10.21228/M8RC75
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000200 AN000201
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent 1290 Agilent 1290
Column Waters Acquity BEH Amide (150 x 2.1mm, 1.7um) Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6530 QTOF Agilent 6530 QTOF
Ion Mode POSITIVE NEGATIVE
Units Peak area Peak area

Chromatography:

Chromatography ID:CH000133
Chromatography Summary:Untargeted LC MS
Instrument Name:Agilent 1290
Column Name:Waters Acquity BEH Amide (150 x 2.1mm, 1.7um)
Column Pressure:300 bar (max)
Column Temperature:40 C
Flow Gradient:0 - 4 min: 0% B; 4-12 min: linear gradient to 45% B; 12-20 min: 45% B
Flow Rate:0.25 mL/min
Injection Temperature:4C
Sample Injection:5 uL
Solvent A:H20 + 5mM NH4OAc + 0.2% Acetic Acid
Solvent B:9:1 Acetonitrile:H20 + 5mM NH4OAc + 0.2% Acetic Acid
Oven Temperature:H20 + 5mM NH4OAc + 0.2% Acetic Acid
Time Program:20 min
Weak Wash Solvent Name:50:50 Methanol:Water
Weak Wash Volume:15 seconds
Target Sample Temperature:Autosampler temperature 4C
Randomization Order:Excel generated
Chromatography Type:HILIC
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