Summary of study ST000143

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000219. The data can be accessed directly via it's Project DOI: 10.21228/M8359Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000143
Study TitleMetabolic analysis of Normal Mouse Lung Fiboblasts with/without TGFbeta treatment (Part 1)
Study TypeGlycolysis/TCA/Nucleotide analysis (tissue/cells)
Study SummaryThis study is a part of series performed for the same researcher through pilot/feasibility grant program, so the publication is relevant reference for other studies, see the project for this study. This specific experiment is a small pilot study to establish method performance, it includes four biological replicas of identical cell cultures after the identical treatment and a single tissue sample.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Email mkachman@umich.edu
Submit Date2015-03-13
Num Groups4
Total Subjects21
Raw Data AvailableYes
Raw Data File Type(s).cd,.cG, .xml, .bin,.stg
Uploaded File Size5.2 G
Analysis Type DetailGC/LC-MS
Release Date2015-03-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8359Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000226 AN000227
Analysis type MS MS
Chromatography type HILIC GC
Chromatography system Agilent 1260 Agilent 7890A
Column Phenomenex Luna NH2 (150 x 1mm, 3um) Agilent DB5-MS (30m × 0.25mm, 0.25um)
MS Type ESI EI
MS instrument type QTOF Single quadrupole
MS instrument name Agilent 6520 QTOF Agilent 5975C
Ion Mode NEGATIVE POSITIVE
Units uM uM

Chromatography:

Chromatography ID:CH000157
Methods ID:AQM020
Methods Filename:QTOF-002-HILIC-35min-1mm.m.zip
Instrument Name:Agilent 1260
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC
  
Chromatography ID:CH001298
Methods ID:AQM011
Methods Filename:ALPHA_KETO_ACIDS-FULL.M.zip
Instrument Name:Agilent 7890A
Column Name:Agilent DB5-MS (30m × 0.25mm, 0.25um)
Chromatography Type:GC
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