Summary of Study ST000237
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000190. The data can be accessed directly via it's Project DOI: 10.21228/M8Q30F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000237 |
Study Title | Quick Comparison of Serum Metabolites in SD Rats of Different Sex by Untargeted UPLC-TOFMS and In-house Software Platform |
Study Type | Biomarker Discovery |
Study Summary | Male (n=8) and female (n=8) SD rats weighing between 220 and 250g were used. On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. Then a blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum. All urine and serum samples were frozen at -80°C prior to analysis. |
Institute | Beijing Institute of Radiation Medicine |
Department | Department of Pharmacology and Toxicology |
Last Name | Liang |
First Name | Qiande |
Address | 27 Taiping Road, Beijing, P.R.China |
liangqiande@yeah.net | |
Submit Date | 2015-08-09 |
Num Groups | 2 |
Total Subjects | 16 |
Raw Data File Type(s) | raw(Waters) |
Analysis Type Detail | LC-MS |
Release Date | 2015-09-09 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN000363 | AN000364 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Waters Acquity | Waters Acquity |
Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
MS Type | ESI | ESI |
MS instrument type | QTOF | QTOF |
MS instrument name | Waters Synapt-HDMS | Waters Synapt-HDMS |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Height | Peak Height |
Chromatography:
Chromatography ID: | CH000265 |
Chromatography Summary: | Untargeted UPLC-TOFMS |
Methods Filename: | 3_CHR_PROT.pdf |
Instrument Name: | Waters Acquity |
Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
Column Temperature: | 24? |
Flow Gradient: | Isocratic 2% B (01 min), linear gradient from 2% to 5% B (12 min), 5% to 12% B (25 min), 12% to 20% B (510 min), 20% to 30% B (1012 min), 30% to 50% B (1213 min), 50% to 100% B (1315 min), isocratic 100% B for 1min, then back to 2% B in 1 min and isocratic 2% B for 3min before next run |
Flow Rate: | 0.5 ml/min |
Injection Temperature: | 24? |
Internal Standard: | Amygdalin |
Sample Injection: | 8?L |
Sampling Cone: | 40V |
Solvent A: | 100% water; 0.1% formic acid |
Solvent B: | 100% acetonitrile; 0.1% formic acid |
Analytical Time: | 20min |
Capillary Voltage: | Positive ion mode:3kV; Negative ion mode: 2.9kV |
Chromatography Type: | Reversed phase |