Summary of Study ST000249

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000201. The data can be accessed directly via it's Project DOI: 10.21228/M8DK5C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000249
Study TitleMeasurement of free amino acid (AA) in response to MYC
Study Typetreatment
Study SummaryThe proposed study will provide us information how the different AAs are affected by MYC. MYC is known to cause increased levels of glutamin due to import and synthesis, but we hypothesize that the overall pool of free AA in the cells is reduced, especially when cells are starved.
Institute
University of Florida
DepartmentSECIM
Last NameSchaub
First NameFranz
EmailFranz.Schaub@moffitt.org
Submit Date2015-09-22
Num Groups2
Total Subjects6
Raw Data AvailableNo
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-09-23
Release Version1
Franz Schaub Franz Schaub
https://dx.doi.org/10.21228/M8DK5C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000393
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Waters AccQ Tag (100 x 2mm,1.7um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units nmol/million cells

Chromatography:

Chromatography ID:CH000279
Chromatography Summary:Amino Acid
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm,1.7um)
Column Temperature:55
Flow Rate:0.7 mL/min
Solvent A:AccQ·Tag Eluent A
Solvent B:accQ·Tag Eluent B
Chromatography Type:Reversed phase
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