Summary of Study ST000259
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000210. The data can be accessed directly via it's Project DOI: 10.21228/M87W2S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000259 |
| Study Title | Metabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in rich LBmc medium |
| Study Type | megaplasmid deletion |
| Study Summary | We wished to evaluate the contribution of pSymA and pSymB towards the utilization of various metabolites in a nutritionally complex environment and to examine how S. meliloti influences its surrounding environment. |
| Institute | McMaster University |
| Department | Department of Biology |
| Last Name | Finan |
| First Name | Turlough |
| Address | Department of Biology, McMaster University, Hamilton, Canada L8S4K1 |
| finan@mcmaster.ca | |
| Phone | (+1)905-525-9140 ext 22932 |
| Submit Date | 2015-09-17 |
| Num Groups | 14 |
| Total Subjects | 82 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-10-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000411 | AN000412 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | HILIC | HILIC |
| Chromatography system | Agilent 1200 RR Series II | Agilent 1200 RR Series II |
| Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| MS Type | ESI | ESI |
| MS instrument type | QTOF | QTOF |
| MS instrument name | Bruker MicrOTOF II | Bruker MicrOTOF II |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized Peak area | Normalized Peak area |
Chromatography:
| Chromatography ID: | CH000291 |
| Methods Filename: | Opt_silicaHILIC.m |
| Instrument Name: | Agilent 1200 RR Series II |
| Column Name: | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
| Column Temperature: | 40C |
| Flow Gradient: | 0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A |
| Flow Rate: | 200 ΔL/min |
| Internal Standard: | L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine |
| Sample Injection: | 2 ΔL |
| Solvent A: | 100% acetonitrile |
| Solvent B: | 100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid |
| Analytical Time: | 24 min |
| Chromatography Type: | HILIC |
