Summary of Study ST000286

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000228. The data can be accessed directly via it's Project DOI: 10.21228/M86014 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000286
Study TitleMouse skeletal myotube chronic low-frequency stimulation
Study TypeElectrical stimulation
Study SummaryCell culture media is collected from mouse myotubes subject to electrical stimulation and control cells with no stimulation.
University of Florida
Last NameVega
First NameRick
Submit Date2015-02-10
Num Groups2
Total Subjects20
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Rick Vega Rick Vega application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN000454 AN000455
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Scientific-Dionex Ultimate 3000: Thermo Scientific-Dionex Ultimate 3000:
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS instrument type Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Units peak height peak height


Chromatography ID:CH000324
Chromatography Comments:UHPLC, Thermo Scientific-Dionex Ultimate 3000: While setting up sequence, ensure that method to be utilized is PFP-metabolomics-neg350-NoBuffer-0-SID and PFP-metabolomics-neg-350-dd-MS2 for negative sequences. For positive sequences utilize PFP-metabolomics-pos-350-0-SID and PFP-metabolomics-pos-350-dd-MS2. Check the lines for air bubbles and purge line if present. Set injection volume to 2uL for positive samples and 4uL for negative samples.
Instrument Name:Thermo Scientific-Dionex Ultimate 3000:
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Chromatography Type:Reversed phase