Summary of Study ST000325

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000262. The data can be accessed directly via it's Project DOI: 10.21228/M8X882 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000325
Study TitleMetabolomic effects of metformin on mouse liver, intestine, and serum
Study SummaryExperiment to test the different metabolomic effects of two different doses of metformin (50mg vs 150 mg). A saline treatment group was used as a control. The effects were measured at the liver, intestine, and serum of the mouse.
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Emailofiehn@ucdavis.edu
Phone(530) 754-8258
Submit Date2015-03-16
Num Groups7
Total Subjects53
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Oliver Fiehn Oliver Fiehn
https://dx.doi.org/10.21228/M8X882
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000518
Analysis type MS
Chromatography type HILIC
Chromatography system Unspecified
Column Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6530 QTOF
Ion Mode POSITIVE
Units Peak height

Chromatography:

Chromatography ID:CH000363
Chromatography Summary:UPLC
Methods Filename:Data_Dictionary_Fiehn_laboratory_HILIC_QTOF_MS.pdf
Instrument Name:Unspecified
Column Name:Waters Acquity BEH Amide (150 x 2.1mm,1.7um)
Column Pressure:200-700 bar
Column Temperature:45 C
Flow Gradient:100% B to 30% B
Flow Rate:0.4 mL/min
Internal Standard:See data dictionary
Retention Time:See data dictionary
Sample Injection:3 uL
Analytical Time:14 min
Capillary Voltage:4500
Time Program:16.75 min
Weak Wash Solvent Name:1:1 ACN:H2O
Weak Wash Volume:5 seconds
Strong Wash Solvent Name:1:1 ACN:H2O
Target Sample Temperature:Autosampler temp 4 C
Randomization Order:Excel
Chromatography Type:HILIC
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