Summary of study ST000337

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000269. The data can be accessed directly via it's Project DOI: 10.21228/M81307 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000337
Study TitleMetabolomics Approach to Allograft Assessment in Liver Transplantation
Study TypeRetrospective analysis of biobanked liver tissue from organ donors
Study SummaryThis pilot study is designed to apply several types of metabolomic analysis for liver allograft assessment with the aim of identifying candidate biomarkers for allograft function and to develop a methodology that could be applied to larger scale studies. The three main categories of metabolomic analysis are reflected in each of the specific aims, including a targeted profiling of central carbon metabolism, open lipidomic and metabolomic profiling for hypothesis generation and MALDI-IMS for tissue-based spatial analysis. Each of these approaches offers potential benefits that could be optimized in a protocol for larger scale studies depending the results of the pilot investigations.
Institute
Ochsner Multi-Organ Transplant Institute
DepartmentSECIM
LaboratoryAbdominal Organ Transplantation
Last NameSeal
First NameJohn
Address1514 Jefferson Highway, New Orleans, LA 70121
EmailJohn.Seal@ochsner.org
Phone504-232-4253
Submit Date2016-01-17
Num Groups3
Total SubjectsTargeted analysis and open profiling: Control group - standard criteria donor (N=15); Experimental group - donation-after-cardiac-death donors (N=10). MALDI-IMS - standard criteria donor (N=10)
Analysis Type DetailLC-MS
Release Date2017-01-17
Release Version1
John Seal John Seal
https://dx.doi.org/10.21228/M81307
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000544 AN000545 AN000546 AN000547 AN000548
Analysis type MS MS MS MS MS
Chromatography type Reversed phase Reversed phase Reversed phase Reversed phase Reversed phase
Chromatography system Agilent 1290 Infinity Agilent 1290 Infinity Agilent 1290 Infinity Agilent 1290 Infinity Thermo Dionex Ultimate 3000
Column Waters AccQ Tag (100 x 2mm, 1.7um) Waters Acquity BEH C18 (50 x 2mm, 1.7um) Thermo Hypercarb (50 x 2.1mm, 3um) Waters Acquity HSS T3 (100 x 2.1mm, 1.8um) Waters Acquity BEH C18 (100 x 2mm, 1.7um)
MS Type ESI ESI ESI ESI ESI
MS instrument type Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole Triple quadrupole
MS instrument name Agilent 6490 QQQ Agilent 6490 QQQ Agilent 6490 QQQ Agilent 6490 QQQ Thermo Quantiva QQQ
Ion Mode POSITIVE POSITIVE POSITIVE POSITIVE POSITIVE
Units nmol/mg dry powder pmol/mg dry powder nmol/mg dry powder nmol/mg dry powder nmol/mg dry powder

Chromatography:

Chromatography ID:CH000381
Chromatography Summary:Amino acid assay
Methods Filename:SB_AA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters AccQ Tag (100 x 2mm, 1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000382
Chromatography Summary:CoA assay
Methods Filename:SB_CoA_Assay.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity BEH C18 (50 x 2mm, 1.7um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000383
Chromatography Summary:NAD-ATP assay
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000384
Chromatography Summary:NADH assay
Methods Filename:SB_NADH_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Waters Acquity HSS T3 (100 x 2.1mm, 1.8um)
Chromatography Type:Reversed phase
  
Chromatography ID:CH000385
Chromatography Summary:Organic acid assay
Methods Filename:SB_OA_Assay.pdf
Instrument Name:Thermo Dionex Ultimate 3000
Column Name:Waters Acquity BEH C18 (100 x 2mm, 1.7um)
Chromatography Type:Reversed phase
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