Summary of Study ST000418

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000327. The data can be accessed directly via it's Project DOI: 10.21228/M8GS39 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000418
Study TitleThe role of CFTR in the regulation of intrinsic defense mechanisms of exocrine secretions
Study SummaryThis study was designed as a pilot to determine if the concentrations of selected ions in salivary gland secretions were influenced by the absence of CFTR on the apical surfaces of glandular cells and ductal epithelia. To this end, five cystic fibrosis children homozygous for Phe508 were to be recruited with their heterozygous non-CF mothers. For adaptation and development of the assays to a microtiter format, saliva samples were collected from non-CF control subjects initially to optimize the assays recognizing that the volumes for analyses would be small. The values obtained with these non-CF control samples were also compared to that of the homozygous and heterozygous CF subjects. As residual volume permits, these samples will be further analyzed for metabolic constituent profiles.
Institute
University of North Carolina
DepartmentRCMRC
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2016-06-15
Num Groups3
Total Subjects22
Num Males10
Num Females12
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2018-06-05
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M8GS39
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000660
Analysis type MS
Chromatography type GC
Chromatography system Leco Pegasus 4D GC
Column Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
MS Type EI
MS instrument type GC x GC-TOF
MS instrument name Leco Pegasus 4D GCxGC TOF
Ion Mode POSITIVE
Units Intensity

Chromatography:

Chromatography ID:CH000477
Instrument Name:Leco Pegasus 4D GC
Column Name:Restek Rtx-5Sil MS (30 x 0.25mm, 0.25um)
Column Pressure:12.8psi
Flow Gradient:constant flow
Flow Rate:1ml/min
Injection Temperature:250C
Retention Index:FAME markers C8-C30
Chromatography Type:GC
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