Summary of Study ST000422
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000330. The data can be accessed directly via it's Project DOI: 10.21228/M8W02Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000422 |
| Study Title | Type 1 Diabetes good glycemic control and controls samples |
| Study Type | Plasma metabolites in T1 diabetes |
| Study Summary | The objective of the study was to determine whether T1D with good glycemic control have persistent abnormalities of metabolites and pathways that exist in T1D with poor glycemic control. |
| Institute | Mayo Clinic |
| Department | Endocrinology |
| Laboratory | Mayo Clinic Metabolomics Resource Core |
| Last Name | Nair |
| First Name | Sreekumaran |
| Address | 200 First Street SW, Rochester, MN 55905 |
| Nair.K@mayo.edu | |
| Phone | 507-285-2415 |
| Submit Date | 2016-07-01 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000667 | AN000668 | AN000669 | AN000670 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Agilent 6220 | Agilent 6220 | Agilent 6220 | Agilent 6220 |
| Column | none | none | none | none |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | TOF | TOF | TOF | TOF |
| MS instrument name | Agilent 6220 TOF | Agilent 6220 TOF | Agilent 6220 TOF | Agilent 6220 TOF |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | intensity | intensity | intensity | intensity |
Chromatography:
| Chromatography ID: | CH000482 |
| Chromatography Summary: | The liquid chromatography platform consisted of an Acquity UPLC system (Waters, Milford, MA). Plasma metabolite separation was achieved using both hydrophilic interaction chromatography (ethylene-bridged hybrid 2.1 × 150 mm, 1.7 μm; Waters) and reversed-phase liquid chromatography C18 (high-strength silica 2.1 × 150 mm, 1.8 μm; Waters). For each column, the run time was 20 min at a flow rate of 400 μL/min. Reverse-phase chromatography was performed using 99% solvent A (5 mmol/L NH4 acetate, 0.1% formic acid, and 1% acetonitrile) to 100% solvent B (95% acetonitrile with 0.1% formic acid). The gradient was 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. Other LC parameters were injection volume 5 μL and column temperature 50°C. Each sample was injected in triplicate with blank injections between each sample. Quality controls and standards were run at the beginning and end of the sequence. |
| Instrument Name: | Agilent 6220 |
| Column Name: | none |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. |
| Flow Rate: | 400 µL/min |
| Solvent A: | 1% acetonitrile/99% water; 0.1% formic acid; 5 mM ammonium acetate |
| Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH000483 |
| Chromatography Summary: | The liquid chromatography platform consisted of an Acquity UPLC system (Waters, Milford, MA). Plasma metabolite separation was achieved using both hydrophilic interaction chromatography (ethylene-bridged hybrid 2.1 × 150 mm, 1.7 μm; Waters) and reversed-phase liquid chromatography C18 (high-strength silica 2.1 × 150 mm, 1.8 μm; Waters). For each column, the run time was 20 min at a flow rate of 400 μL/min. Reverse-phase chromatography was performed using 99% solvent A (5 mmol/L NH4 acetate, 0.1% formic acid, and 1% acetonitrile) to 100% solvent B (95% acetonitrile with 0.1% formic acid). The gradient was 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. Other LC parameters were injection volume 5 μL and column temperature 50°C. Each sample was injected in triplicate with blank injections between each sample. Quality controls and standards were run at the beginning and end of the sequence. |
| Instrument Name: | Agilent 6220 |
| Column Name: | none |
| Column Temperature: | 50 |
| Flow Gradient: | 0 min, 0% B; 1 min, 0% B; 3 min, 5% B; 13.0 min, 100% B; 16 min, 100% B; 16.5 min, 0% B; and 20 min, 0% B. |
| Flow Rate: | 400 µL/min |
| Solvent A: | 99% water/1% acetonitrile; 0.1% formic acid; 5 mM ammonium acetate |
| Solvent B: | 95% acetonitrile/5% water; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
