Summary of Study ST000445
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000343. The data can be accessed directly via it's Project DOI: 10.21228/M8JS49 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000445 |
| Study Title | Follicular fluid lipidomics reveals lipid alterations by LH addition during IVF cycles |
| Study Summary | Purpose Ovulation induction protocols are key components for performing assisted reproduction treatments successfully. The objective of the present study was to estimate how LH addition to controlled ovarian stimulation protocols may affect the follicular fluid lipid profile of women undergoing in vitro fertilization treatment. Methods We conducted the study using 28 self-paired samples, 14 per group. The patients received FSH during their first cycle of ovarian stimulation (FSH group). If treatment did not result in pregnancy, the same patients returned for a new cycle and received stimulus with the addition of LH to the previous protocol (Low-dose-LH group). Lipidomics analysis was performed by UPLC-MSE mass spectrometry. Potential lipid biomarkers were identified by the software Progenesis QI. Statistical analysis was performed using the SPSS 18.0 and MetaboAnalyst 2.0 software. |
| Institute | Universidade Federal de Sao Paulo |
| Department | Surgery |
| Laboratory | Centro de Pesquisa em Urologia |
| Last Name | Da Costa |
| First Name | Livia |
| Address | Rua Embau 231 - Vila Clementino, Sao Paulo, Sao Paulo, 04039060, Brazil |
| liviadovale@hotmail.com | |
| Phone | 551138074062 |
| Submit Date | 2016-07-25 |
| Num Groups | 2 |
| Total Subjects | 28 |
| Num Females | 14 |
| Study Comments | The groups consists of the same 14 women submitted to two different controlled ovarian stimulation protocols (FSH or LH group) |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-09-23 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000696 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity I-Class |
| Column | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 S QTOF |
| Ion Mode | POSITIVE |
| Units | m/z intensity |
Chromatography:
| Chromatography ID: | CH000504 |
| Chromatography Summary: | Reversed-phase analysis was performed on a Waters Ultra Performance liquid chromatography Acquity I-Class system using an Acquity HSS T3 2.10 x 100 mm column (Waters, Milford, USA). The column was maintained at 55 °C. The lipid extracts were analyzed by UPLC-MSE by injecting 10 μl of each sample dissolved in 1 mL of water:acetonitrile:2-propanol (H2O:ACN:2-propanol; 1:1:2, v:v:v) (Merck, Darmstadt, Germany). The chromatographic run was carried out in gradient mode with a flow rate of 0.5 mL min-1 with initial composition of 50% solvent B (ACN:2-propanol; 10:90) in solvent A (H2O:ACN; 40:60, v:v + 10 mM ammonium acetate (NH4Ac) (Sigma-Aldrich, Saint Louis, USA), maintained for 0 to 0.5 min. The gradient composition was changed linearly from 50% to 100% solvent B between 0.5 to 4.5 minutes, returning to the initial composition of 50% B in 4.6 minutes, kept to 5.0 minutes. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 55ºC |
| Flow Rate: | 0.5 mL.min-1 |
| Solvent A: | 40% water/60% acetonitrile; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/90% isopropanol |
| Chromatography Type: | Reversed phase |
