Summary of study ST000450

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000348. The data can be accessed directly via it's Project DOI: 10.21228/M82K58 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Download all metabolite data  |  Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data
Study IDST000450
Study TitleMetabolic features of chronic fatigue syndrome
Study TypePlasma metabolomic profiling
Study SummaryThis targeted metabolomic analysis was performed on plasma samples from 39 normal controls (n=18 men and 21 women) and 45 subjects ((n = 22 men and 23 women) who met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria.
Institute
University of California, San Diego
DepartmentThe Mitochondrial and Metabolic Disease Center
Last NameNaviaux
First NameRobert
Address214 Dikinson Street, CTF-C102, San Diego, CA, 92103
Emailmaviaux@ucsd.edu
Phone619-993-2904
Submit Date2016-08-11
Num Groups2 groups for men (control and CFS) and 2 groups for women (control and CFS)
Total Subjects84
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Robert Naviaux Robert Naviaux
https://dx.doi.org/10.21228/M82K58
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Combined analysis:

Analysis ID AN000705 AN000706
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Shimadzu 20AD Shimadzu 20AD
Column Shodex NH2P-40 2E (250 mm × 2.0 mm) Shodex NH2P-40 2E (250 mm × 2.0 mm)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 5500 QTrap
Ion Mode POSITIVE NEGATIVE
Units AUC AUC

Chromatography:

Chromatography ID:CH000510
Chromatography Summary:HILIC
Instrument Name:Shimadzu 20AD
Column Name:Shodex NH2P-40 2E (250 mm × 2.0 mm)
Column Pressure:920~2600 psi
Column Temperature:25C
Flow Gradient:0-3.5 min: 95%B, 3.6-8 min: 85% B, 8.1-13 min: 75% B, 13.5–35 min: 0% B, 36–46 min: 95% B, 46.1 min: end.
Flow Rate:0.2 ml/min
Solvent A:95% water with 20 mM (NH4)2CO3, 5% acetonitrile, and 38 mM NH4OH, final pH 9.75
Solvent B:100% acetonitrile
Analytical Time:46.1 min
Oven Temperature:25C
Sample Loop Size:10 ul
Sample Syringe Size:100 ul
Chromatography Type:HILIC
  logo