Summary of Study ST000473
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000361. The data can be accessed directly via it's Project DOI: 10.21228/M8CW21 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000473 |
| Study Title | Effect of the chemical environment on the degradation of nucleotide triphosphates |
| Study Type | Endpoint measurement |
| Study Summary | The influence of particular groups of compounds/metabolites, mainly comprising major central carbon metabolites including amino acids, organic acids, sugar phosphates, coenzymes, etc, on the degradation profiles of nucleotide triphosphates extracted under typical boiling ethanol conditions was evaluated. |
| Institute | University of Groningen |
| Department | Analytical Biochemistry |
| Last Name | Bischoff |
| First Name | Rainer |
| Address | Antonius Deusinglaan 1 (XB20), Building 3226, room 601, 9713 AV Groningen, The Netherlands |
| r.p.h.bischoff@rug.nl | |
| Phone | NA |
| Submit Date | 2016-09-10 |
| Publications | Gil, A., Siegel, D., Bonsing-Vedelaar, S. et al. The degradation of nucleotide triphosphates extracted under boiling ethanol conditions is prevented by the yeast cellular matrix. Metabolomics (2017) 13:1. doi:10.1007/s11306-016-1140-4 |
| Raw Data Available | Yes |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-04-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000737 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Waters Acquity |
| Column | Phenomenex Luna NH2 (100 x 2.0mm,3um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters Synapt G2 Si QTOF |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000530 |
| Chromatography Summary: | Untargeted HILIC Method |
| Instrument Name: | Waters Acquity |
| Column Name: | Phenomenex Luna NH2 (100 x 2.0mm,3um) |
| Column Temperature: | 20C |
| Flow Gradient: | 30% eluent A to 99% eluent A in 8 min, followed by isocratic elution at 99% eluent A until 14 min. A conditioning cycle of 6 min with the initial proportions of eluents A and B was performed prior to the next analysis. |
| Flow Rate: | 0.25 mL/min |
| Sample Injection: | 10 µL |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 20 min |
| Chromatography Type: | HILIC |
