Summary of Study ST000553

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000405. The data can be accessed directly via it's Project DOI: 10.21228/M8Q01S This work is supported by NIH grant, U2C- DK119886.

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Study IDST000553
Study TitleCHEAR UT Urine and Plasma Pool Characterization
Study SummaryAnalyze CHEAR UT urine and plasma pool in six replicates
Institute
Mount Sinai
Last NameAndra
First NameSyam
AddressMount Sinai
Emailsyam.andra@mssm.edu
Phone212-241-7369
Submit Date2017-02-14
Chear StudyYes
Analysis Type DetailLC-MS
Release Date2024-12-31
Release Version1
Syam Andra Syam Andra
https://dx.doi.org/10.21228/M8Q01S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000845 AN000846 AN000847 AN000848
Analysis type MS MS MS MS
Chromatography type Reversed phase Reversed phase HILIC HILIC
Chromatography system Agilent 1290 Infinity II Agilent 1290 Infinity II Agilent 1290 Infinity II Agilent 1290 Infinity II
Column Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) EMD Millipore ZIC-HILIC (100 x 2.1mm,3.5um) EMD Millipore ZIC-HILIC (100 x 2.1mm,3.5um)
MS Type ESI ESI ESI ESI
MS instrument type QTOF QTOF QTOF QTOF
MS instrument name Agilent 6550 QTOF Agilent 6550 QTOF Agilent 6550 QTOF Agilent 6550 QTOF
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Peak Area Peak Area Peak Area Peak Area

Chromatography:

Chromatography ID:CH000604
Chromatography Summary:Metabolites were separated using a C18 column, Zorbax Eclipse Plus C18 (2.1 x 100 mm, 1.8μm particle size), mobile phase A: water and mobile phase B: IPA/ACN(90:10) both with 0.1% HCOOH at a flow rate of 400μL/min injecting 2μL of extracts on a 1290 Infinity II LC System (Agilent Technologies).
Instrument Name:Agilent 1290 Infinity II
Column Name:Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)
Flow Rate:400µL/min
Solvent A:100% water; 0.1% formic acid
Solvent B:90% isopropanol/10% acetonitrile; 0.1% formic acid
Chromatography Type:Reversed phase
  
Chromatography ID:CH000605
Chromatography Summary:Polar metabolites were analyzed using a EMD Millipore ZIC-HILIC (100 mm x 2.1 mm ID, 3.5μm) column, mobile phase A: water with 0.1% formic acid and B: Acetonitrile with 0.1% formic acid at a flow rate 300μL/min. 2μL of extract were injected on a 1290 Infinity II LC System (Agilent Technologies). For the negative ionization mode, mobile phase A was 10mM ammonium acetate and B was acetonitrile.
Instrument Name:Agilent 1290 Infinity II
Column Name:EMD Millipore ZIC-HILIC (100 x 2.1mm,3.5um)
Flow Rate:300µL/min
Solvent A:Pos mode:100% water; 0.1% formic acid, Neg mode:100% water; 10 mM ammonium acetate
Solvent B:Pos mode:100% acetonitrile; 0.1% formic acid, Neg mode:100% acetonitrile
Chromatography Type:HILIC
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