Summary of Study ST000561
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000412. The data can be accessed directly via it's Project DOI: 10.21228/M8SS33 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000561 |
| Study Title | Exploring the link between genotype, phenotype and metabolome for tomato seed quality attributes |
| Study Type | Tomato Seed Metabolites Profiling (dry seed and 6 hour imbibed seeds comparision) |
| Study Summary | In this study the F8 population of 100 Recombinant Inbred Lines (RILs) obtained from a cross between Solanum lycopersicum X Solanum pimpinellifolium were intelligently allocated to two sub-populations optimized for the distribution of parental alleles using the R-procedure DesignGG (Li et al., 2009; Joosen et al., 2013); hence 50 RIL lines were used for dry seeds and 50 lines for 6h imbibed seeds |
| Institute | Wageningen University & Research |
| Department | Plant Physiology |
| Laboratory | Wageningen Seed Lab, Lab |
| Last Name | Ligterink |
| First Name | Wilco |
| Address | Droevendaalsesteeg 1, NL-6708 PB Wageningen, The Netherlands |
| wilco.ligterink@wur.nl | |
| Phone | 31 317 48 28 09 |
| Submit Date | 2017-02-07 |
| Num Groups | 1 |
| Total Subjects | 100 |
| Publications | Metabolomic analysis of tomato seed germination, Metabolomics DOI: 10.1007/s11306-017-1284-x |
| Analysis Type Detail | GC-MS |
| Release Date | 2017-10-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000862 | AN000863 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | GC | GC |
| Chromatography system | Agilent 6890N | Agilent 6890N |
| Column | Zebron Z-guard guard GC Cap 5m x 0.1mm | Zebron Z-guard guard GC Cap 5m x 0.1mm |
| MS Type | EI | EI |
| MS instrument type | GC x GC-TOF | GC x GC-TOF |
| MS instrument name | Agilent | Agilent |
| Ion Mode | POSITIVE | POSITIVE |
| Units | Peak Area | Peak Area |
Chromatography:
| Chromatography ID: | CH000613 |
| Chromatography Summary: | Untargetted GC-TOF-MS |
| Methods Filename: | SL_COL_PROT.pdf |
| Chromatography Comments: | Detector voltage: 1600 |
| Instrument Name: | Agilent 6890N |
| Column Name: | Zebron Z-guard guard GC Cap 5m x 0.1mm |
| Column Temperature: | 300°C |
| Flow Rate: | 1ml/min |
| Injection Temperature: | 70°C |
| Internal Standard: | Ribitol |
| Retention Index: | 1000-3100 |
| Retention Time: | 300-1800 sec |
| Sample Injection: | 2µL |
| Solvent A: | 20mg 0-methylhydroxylamine hydrochloride/ml pyridine |
| Solvent B: | MSTFA (derivatization agent) |
| Analytical Time: | 1800sec |
| Oven Temperature: | 70->300 |
| Running Voltage: | 1600 |
| Time Program: | 1800 sec |
| Transferline Temperature: | 270°C |
| Washing Buffer: | CHCl3 |
| Target Sample Temperature: | 40°C |
| Sample Syringe Size: | 25°C |
| Randomization Order: | Yes |
| Chromatography Type: | GC |
