Summary of study ST000591

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000432. The data can be accessed directly via it's Project DOI: 10.21228/M86W3V This work is supported by NIH grant, U2C- DK119886.

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Study IDST000591
Study TitleMetablomic profiling in acc1-5 mutant and wild type arabidiopsis
Study SummaryThis experiment tests the metabolic consequence of a mutation at the ACC1 gene (At1g36160). The allele of acc1-5 bearing an EMS mutation, which cause a single amino acid substitution from aspartic acid to asparagine. Seedlings from both the acc1-5 mutant and the wild type were harvested and analyzed via HILIC LC-MS. Of particular interest are metabolites which would be affected by depletion of malonyl-CoA pools (flavenoids) and primary metabolites.
Institute
Agriculture and Agri-Food Canada
DepartmentLondon Research and Development Centre
LaboratoryRenaud
Last NameRenaud
First NameJustin
Address1391 Sandford street, London, Ontario, Canada
Emailjustin.renaud@agr.gc.ca
Phone519-953-6698
Submit Date2017-03-12
PublicationsChen, Chen, et al. "Cytosolic acetyl-CoA promotes histone acetylation predominantly at H3K27 in Arabidopsis." Nature Plants (2017): 1.
Raw Data AvailableYes
Raw Data File Type(s).raw
Analysis Type DetailLC-MS
Release Date2017-10-25
Release Version1
Justin Renaud Justin Renaud
https://dx.doi.org/10.21228/M86W3V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000906
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent 1290
Column SeQuant ZIC- pHILIC (100 x 2.1mm, 3.5um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000643
Chromatography Summary:Samples were resolved using HILIC column
Instrument Name:Agilent 1290
Column Name:SeQuant ZIC- pHILIC (100 x 2.1mm, 3.5um)
Column Temperature:30C
Flow Gradient:87% B for 5 minutes, decreased to 55% over 8 minutes and held for 4 minutes before returning to 87% over 3 minute
Flow Rate:0.3 ml min-1
Internal Standard:13C6 phenylalanine internal standard
Solvent A:5 mM ammonium acetate, pH = 4.00
Solvent B:B = 90% acetonitrile, 0.1% formic acid
Chromatography Type:HILIC
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